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Int J Syst Evol Microbiol 59 (2009), 2010-2014; DOI  10.1099/ijs.0.007567-0
© 2009 International Union of Microbiological Societies

Lactobacillus oeni sp. nov., from wine

Rosario Mañes-Lázaro1, Sergi Ferrer1, Ramón Rosselló-Mora2 and Isabel Pardo1

1 ENOLAB – Laboratorio de Microbiología Enológica, Departamento de Microbiología y Ecología, Facultad de Ciencias Biológicas, Universidad de Valencia, Dr Moliner 50, E-46100 Burjasot, Valencia, Spain
2 Institut Mediterrani d'Estudis Avançats (CSIC-UIB), E-07190 Esporles, Mallorca, Spain

Correspondence
Isabel Pardo
Isabel.Pardo{at}uv.es

Ten Lactobacillus strains, previously isolated from different Bobal grape wines from the Utiel-Requena Origin Denomination of Spain, were characterized phylogenetically, genotypically and phenotypically. The 16S rRNA genes were sequenced and phylogenetic analysis showed that they form a tight phylogenetic clade that is closely related to reference strains Lactobacillus satsumensis NRIC 0604T, ‘Lactobacillus uvarum’ 8 and Lactobacillus mali DSM 20444T. DNA–DNA hybridization results confirmed the separation of the strains from other Lactobacillus species. Genotypically, the strains could be differentiated from their closest neighbours by 16S amplified rDNA restriction analysis and random amplified polymorphic DNA patterns. The strains were Gram-staining-positive, facultatively anaerobic rods that did not exhibit catalase activity. Phenotypically, they could be distinguished from their closest relatives by several traits such as their inabilities to grow at pH 3.3, to ferment sucrose, amygdalin and arbutin or to hydrolyse aesculin. The characteristics of the ten wine isolates suggest that they represent a novel species, for which the name Lactobacillus oeni sp. nov. is proposed. The type strain is 59bT (=CECT 7334T =DSM 19972T).

Abbreviations: ARDRA, amplified rDNA restriction analysis; RAPD, random amplified polymorphic DNA

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains 59bT, 54, 59c, 80, 81, 82, 103, 209g, 376 and 420 are AY681127 and EU821345–EU821353, respectively.

Phylogenetic trees based on 16S rRNA gene sequences and constructed using maximum-parsimony and neighbour-joining methods are available as supplementary figures with the online version of this paper.






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