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Dokdonella ginsengisoli sp. nov., isolated from soil from a ginseng field, and emended description of the genus Dokdonella

¹ Department of Biology and Medicinal Science, Pai Chai University, 14 Yeonja-1-Gil, Seo-Gu, Daejeon 302-735, Republic of Korea
² Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea
³ Insect Resources Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 52 Oeundong, Yusong-gu, Daejeon 305-333, Republic of Korea
⁴ Department of Oriental Medicinal Material and Processing College of Life Science, Kyung Hee University, 1 Seocheon, Kihung Yongin, Kyunggi 449-701, Republic of Korea

Correspondence
Wan-Taek Im
wandra{at}kaist.ac.kr
Sung-Taik Lee
e_stlee{at}kaist.ac.kr

A Gram-negative, aerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated Gsoil 191^T, was isolated from a soil sample from a ginseng field in Pocheon Province, South Korea, and was characterized taxonomically by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain Gsoil 191^T belongs to the family Xanthomonadaceae and is related to Dokdonella fugitiva LMG 23001^T (97.8 % sequence similarity) and Dokdonella koreensis KCTC 12396^T (96.9 %). The G+C content of the genomic DNA was 68.7 mol%. The major respiratory quinone was Q-8 and the major fatty acids were iso-C_{17 : 1}9c (30.6 %), iso-C_{17 : 0} (21.6 %) and iso-C_{15 : 0} (13.0 %), supporting the affiliation of strain Gsoil 191^T to the genus Dokdonella. DNA–DNA hybridization experiments showed that the DNA–DNA relatedness values between strain Gsoil 191^T and its closest phylogenetic neighbours were below 40 %. The results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain Gsoil 191^T from recognized species of the genus Dokdonella. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 191^T represents a novel species of the genus Dokdonella, for which the name Dokdonella ginsengisoli sp. nov. is proposed. The type strain is Gsoil 191^T (=KCTC 12564^T=DSM 17954^T=CCUG 52462^T).

A transmission electron micrograph of a cell of strain Gsoil 191^T and two-dimensional thin-layer chromatograms of the polar lipids of the strain are available as supplementary material with the online version of this paper.