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Int J Syst Evol Microbiol 59 (2009), 1941-1946; DOI  10.1099/ijs.0.003368-0
© 2009 International Union of Microbiological Societies

Ideonella azotifigens sp. nov., an aerobic diazotroph of the Betaproteobacteria isolated from grass rhizosphere soil, and emended description of the genus Ideonella

Jesse D. Noar and Daniel H. Buckley

Department of Crop and Soil Sciences, Cornell University, Ithaca, NY 14853, USA

Correspondence
Daniel H. Buckley
dhb28{at}cornell.edu

Strain 1a22T, a nitrogen-fixing bacterium, was isolated from soil associated with the rhizosphere of a perennial grass growing in a fallow agricultural field in Ithaca, New York, USA. Analysis of the 16S rRNA gene sequence placed the strain in the Rubrivivax–Roseateles–Leptothrix–Azohydromonas–Aquincola–Ideonella branch of the Betaproteobacteria and the closest characterized relative was the type strain of Ideonella dechloratans (97.7 % 16S rRNA sequence similarity). Cells of strain 1a22T were Gram-negative, motile, straight rods, which formed polyhydroxybutyrate-like granules and were positive for oxidase and weakly positive for catalase. Cells were chemo-organotrophic, unable to grow by reduction of chlorate or nitrate and grew exclusively through aerobic respiration. Growth with mannitol on N-free solid media caused the strain to produce copious amounts of slime. The G+C content of the genomic DNA was 67.4 mol%. The major cellular fatty acids were C16 : 1 cis-9 and C16 : 0 and cells contained significant amounts of the hydroxy fatty acids C10 : 0 3-OH, C12 : 0 2-OH and C12 : 0 3-OH. Based on DNA–DNA hybridization studies, 16S rRNA gene sequence analysis, fatty acid analysis, and morphological and physiological characteristics, strain 1a22T represents a novel species in the genus Ideonella, for which the name Ideonella azotifigens sp. nov. is proposed. The type strain of Ideonella azotifigens is 1a22T (=JCM 15503T=DSM 21438T).

Abbreviations: PW-PCR, plate-wash PCR

The GenBank/EMBL/DDBJ accession number for the 16S rRNA and nifH gene sequences of Ideonella azotifigens strain 1a22T are EU542576 and EU542577, respectively.






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