HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Figures All Versions of this Article: ijs.0.000463-0v1 59/8/1908    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Google Scholar Articles by Mancinelli, R. L. Articles by Stan-Lotter, H. PubMed PubMed Citation Articles by Mancinelli, R. L. Articles by Stan-Lotter, H. Agricola Articles by Mancinelli, R. L. Articles by Stan-Lotter, H.

Halorubrum chaoviator sp. nov., a haloarchaeon isolated from sea salt in Baja California, Mexico, Western Australia and Naxos, Greece

¹ SETI Institute, 515 N. Whisman Road, Mountain View, CA 94043, USA
² Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain
³ Division of Molecular Biology, Department of Microbiology, University of Salzburg, Billrothstraße 11, A-5020 Salzburg, Austria
⁴ Dr.-Petter-Str. 20, A-5020 Salzburg, Austria
⁵ Center for Gene Research, Nagoya University, Nagoya 464-8602, Japan

Correspondence
Helga Stan-Lotter
helga.stan-lotter{at}sbg.ac.at

Three halophilic isolates, strains Halo-G*^T, AUS-1 and Naxos II, were compared. Halo-G* was isolated from an evaporitic salt crystal from Baja California, Mexico, whereas AUS-1 and Naxos II were isolated from salt pools in Western Australia and the Greek island of Naxos, respectively. Halo-G*^T had been exposed previously to conditions of outer space and survived 2 weeks on the Biopan facility. Chemotaxonomic and molecular comparisons suggested high similarity between the three strains. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that the strains clustered with Halorubrum species, showing sequence similarities of 99.2–97.1 %. The DNA–DNA hybridization values of strain Halo-G*^T and strains AUS-1 and Naxos II are 73 and 75 %, respectively, indicating that they constitute a single species. The DNA relatedness between strain Halo-G*^T and the type strains of 13 closely related species of the genus Halorubrum ranged from 39 to 2 %, suggesting that the three isolates constitute a different genospecies. The G+C content of the DNA of the three strains was 65.5–66.5 mol%. All three strains contained C₂₀C₂₀ derivatives of diethers of phosphatidylglycerol, phosphatidylglyceromethylphosphate and phosphatidylglycerolsulfate, together with a sulfated glycolipid. On the basis of these results, a novel species that includes the three strains is proposed, with the name Halorubrum chaoviator sp. nov. The type strain is strain Halo-G*^T (=DSM 19316^T =NCIMB 14426^T =ATCC BAA-1602^T).

Phase-contrast micrographs of cells of strains Halo-G*^T and Naxos II and 2D TLC of the polar lipids of strains Halo-G*^T, AUS-1 and Naxos II are available as supplementary material with the online version of this paper.