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Int J Syst Evol Microbiol 59 (2009), 1813-1817; DOI  10.1099/ijs.0.007765-0
© 2009 International Union of Microbiological Societies

Pseudozyma pruni sp. nov., a novel ustilaginomycetous anamorphic fungus from flowers in Taiwan

Guey-Yuh Liou, Yu-Hui Wei, Shie-Jea Lin, Chiou-Yen Wen and Fwu-Ling Lee

Bioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute (FIRDI), PO Box 246, Hsinchu 30062, Taiwan, ROC

Correspondence
Fwu-Ling Lee
fll{at}firdi.org.tw

Two ustilaginomycetous anamorphic strains were isolated from flowers in Taiwan. Phylogenetic analysis based on the combined rRNA gene sequence of internal transcribed spacer 1 (ITS1)–5.8S–ITS2 and large-subunit D1/D2 domains indicated that the closest recognized species was Pseudozyma fusiformata. The results of DNA–DNA hybridization and physiological characteristics showed that the two strains represent a novel species within the genus Pseudozyma. The name Pseudozyma pruni sp. nov. is proposed, with FIRDI 005T (=BCRC 34227T =CBS 10937T) as the type strain.

Abbreviations: ITS, internal transcribed spacer; LSU, large subunit

The GenBank/EMBL/DDBJ accession numbers for the rRNA gene sequences of the ITS1–5.8S–ITS2 and LSU D1/D2 domains of Pseudozyma pruni FIRDI 005T are EU379942 and EU379943, respectively.

A maximum-parsimony phylogenetic tree based on the combined sequences of the rRNA gene ITS region and LSU D1/D2 domain of the novel species and related species, and a table showing the results of DNA–DNA hybridization, are available as supplementary material with the online version of this paper.






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Copyright © 2009 by the International Union of Microbiological Societies.