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Marinobacter lacisalsi sp. nov., a moderately halophilic bacterium isolated from the saline-wetland wildfowl reserve Fuente de Piedra in southern Spain

¹ Department of Microbiology, Faculty of Pharmacy, University of Granada, Campus Universitario de Cartuja s/n, 18071 Granada, Spain
² Department of Natural Sciences, Faculty of Sciences, University of Mentouri, Constantine, Algeria
³ Microbiology Laboratories, Department of Biology, Imperial College at Wye, Wye Ashford, Kent TN25 5AH, UK

Correspondence
Mercedes Monteoliva-Sánchez
mmonteol{at}ugr.es

A Gram-negative, non-spore-forming, motile, moderately halophilic, aerobic, rod-shaped bacterium, designated strain FP2.5^T, was isolated from the inland hypersaline lake Fuente de Piedra, a saline-wetland wildfowl reserve located in the province of Málaga in southern Spain. Strain FP2.5^T was subjected to a polyphasic taxonomic study. It produced colonies with a light-yellow pigment. Strain FP2.5^T grew at salinities of 3–15 % (w/v) and at temperatures of 20–40 °C. The pH range for growth was 5–9. Strain FP2.5^T was able to utilize various organic acids as sole carbon and energy source. Its major fatty acids were C_{16 : 0}, C_{18 : 1}9c and C_{16 : 1}9c. The DNA G+C content was 58.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain FP2.5^T appeared to be a member of the genus Marinobacter and clustered closely with the type strains of Marinobacter segnicrescens, Marinobacter bryozoorum and Marinobacter gudaonensis (levels of 16S rRNA gene sequence similarity of 98.1, 97.4 and 97.2 %, respectively). However, DNA–DNA relatedness between the new isolate and the type strains of its closest related Marinobacter species was low; levels of DNA–DNA relatedness between strain FP2.5^T and M. segnicrescens LMG 23928^T, M. bryozoorum DSM 15401^T and M. gudaonensis DSM 18066^T were 36.3, 32.1 and 24.9 %, respectively. On the basis of phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness data, strain FP2.5^T is considered to represent a novel species of the genus Marinobacter, for which the name Marinobacter lacisalsi sp. nov. is proposed. The type strain is FP2.5^T (=CECT 7297^T=LMG 24237^T).

A maximum-parsimony phylogenetic tree based on 16S rRNA gene sequences showing the relationship between strain FP2.5^T and the type strains of other species of the genus Marinobacter is available as supplementary material with the online version of this paper.