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Int J Syst Evol Microbiol 59 (2009), 1650-1655; DOI  10.1099/ijs.0.005967-0
© 2009 International Union of Microbiological Societies

Enterobacter oryzae sp. nov., a nitrogen-fixing bacterium isolated from the wild rice species Oryza latifolia

Guixiang Peng1, Wu Zhang2, Huifen Luo2, Hongwei Xie1, Weihao Lai2 and Zhiyuan Tan2

1 College of Resources and Environment, South China Agricultural University, Guangzhou 510642, PR China
2 Provincial Key Lab of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou 510642, PR China

Correspondence
Zhiyuan Tan
zytan{at}scau.edu.cn

Twelve facultatively anaerobic, endophytic diazotrophs were isolated from surface-sterilized roots of the wild rice species Oryza latifolia and characterized by phenotypic and molecular methods. Six isolates were grouped together as group A by phenotypic characters, and this grouping was confirmed by SDS-PAGE whole-cell protein patterns and insertion sequence-based PCR (IS-PCR) methods. Phylogenetic analysis of the 16S rRNA gene sequence indicated that group A, represented by strain Ola 51T, is closely related to Enterobacter radicincitans D5/23T (98.9 % similarity, except that E. radicincitans D5/23T has a 70 bp insertion) and Enterobacter cloacae (98.0 % similarity to the type strain). rpoB gene sequence analysis also showed strain Ola 51T has the highest sequence similarity to E. radicincitans DSM 16656T (98.3 %), but supported the distinct position. Biological and biochemical tests, protein patterns, genomic DNA fingerprinting, antibiotic resistance and comparison of cellular fatty acids showed differences among group A, E. radicincitans DSM 16656T and E. cloacae ATCC 13047T. DNA–DNA hybridization distinguished strain Ola 51T from closely phylogenetically related Enterobacter species. Based on these data, the novel species Enterobacter oryzae sp. nov. is proposed, with strain Ola 51T (=LMG 24251T =CGMCC 1.7012T) as the type strain.

The GenBank/EMBL/DDBJ accession numbers for the partial 16S rRNA gene sequences of strains Ola 50, Ola 51T and Ola 01 are respectively EF488758–EF488760 and the accession number for the partial rpoB gene sequence of strain Ola 51T is EU249553.

A comparison of the biological and biochemical characteristics of strain Ola 51T with those of related strains, detailed DNA–DNA hybridization results, a transmission electron micrograph of cells of strain Ola 51T, comparisons of IS-PCR and whole-cell protein SDS-PAGE profiles of selected novel strains and related strains and an rpoB gene sequence-based neighbour-joining tree are available as supplementary material with the online version of this paper.






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