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Int J Syst Evol Microbiol 59 (2009), 1645-1649; DOI  10.1099/ijs.0.004283-0
© 2009 International Union of Microbiological Societies

Vibrio azureus sp. nov., a luminous marine bacterium isolated from seawater

Susumu Yoshizawa1, Minoru Wada1, Kumiko Kita-Tsukamoto1, Eiko Ikemoto1, Akira Yokota2 and Kazuhiro Kogure1

1 Ocean Research Institute, The University of Tokyo, 1-15-1, Minamidai, Nakano-Ku, Tokyo 164-8639, Japan
2 Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo 113-0032, Japan

Correspondence
Susumu Yoshizawa
y_susumu{at}nenv.k.u-tokyo.ac.jp

Two luminous marine bacterial strains, LC2-005T and LC2-102, were isolated from seawater at Kuroshio Region and Sagami Bay in Japan, respectively. These bacteria were Gram-negative, oxidase-positive, catalase-positive, motile and rod-shaped. On the basis of 16S rRNA gene sequence analysis, strains LC2-005T and LC2-102 formed a cluster within the Vibrio harveyi species group. However, multilocus sequence analysis using five loci (pyrH, ftsZ, mreB, gyrB and gapA) and DNA–DNA hybridization experiments indicated that these strains were distinct from the currently known Vibrio species. Additionally, these strains differ from related Vibrio species in utilization of glucose, mannitol, inositol, sorbitol, rhamnose, sucrose, melibiose and arabinose, production of lysine decarboxylase, ornithine decarboxylase, tryptophan deaminase, esterase (C4), lipase (C4), chymotrypsin, acid phosphatase, {alpha}-glucosidase, β-glucosidase and N-acetyl-β-glucosaminidase and the ability to reduce nitrate to nitrite. The major fatty acids were C15 : 0 iso 2-OH and/or C16 : 1{omega}7c, C16 : 0, C18 : 1{omega}7c and C14 : 0. The DNA G+C contents of strains LC2-005T and LC2-102 were 45.2 and 45.5 mol%, respectively. On the basis of the polyphasic taxonomic evidence presented in this study, it can be concluded that strains LC2-005T and LC2-102 belong to the same genospecies and represent a novel species of the genus Vibrio, for which the name Vibrio azureus sp. nov. is proposed. The type strain is LC2-005T (=NBRC 104587T =KCTC 22352T).

Abbreviations: MLSA, multilocus sequence analysis

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, gyrB, pyrH, ftsZ, mreB and gapA gene sequences of strains NBRC 104587T and NBRC 104588 determined in this study are AB428897–AB428908 (see Supplementary Table S1, available with the online version of this paper).

Two supplementary tables and eight supplementary figures are available with the online version of this paper.






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