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Pontibacillus halophilus sp. nov., a moderately halophilic bacterium isolated from a sea urchin

¹ Key Laboratory for Conservation and Utilization of Plant Resources of Hunan Province, College of Biology and Environmental Sciences, Jishou University, Jishou, Hunan 416000, PR China
² The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China
³ Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China
⁴ Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong 510301, PR China

Correspondence
Wen-Jun Li
wjli{at}ynu.edu.cn
Yi-Guang Chen
mchenjsu{at}yahoo.com.cn

A Gram-positive-staining, moderately halophilic, strictly aerobic, catalase- and oxidase-positive, rod-shaped bacterium, designated strain JSM 076056^T, was isolated from a sea urchin collected from the South China Sea. Cells were motile by means of peritrichous flagella and formed ellipsoidal endospores lying in subterminal swollen sporangia. Strain JSM 076056^T was able to grow at salinities of 2–25 % (w/v) total salts and at pH 6.0–10.0 and 15–40 °C; optimum growth was observed with 5–10 % (w/v) total salts and at pH 7.0–8.0 and 25–30 °C. It was incapable of growing with NaCl as the sole salt. meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The major cellular fatty acids were iso-C_{15 : 0}, iso-C_{16 : 0}, iso-C_{14 : 0,} anteiso-C_{15 : 0} and C_{16 : 1}7c alcohol. The predominant respiratory quinone was MK-7 and the genomic DNA G+C content was 45.5 mol%. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JSM 076056^T belonged to the family Bacillaceae and was related most closely to the type strains of the two recognized species of the genus Pontibacillus, namely Pontibacillus chungwhensis BH030062^T (96.4 % sequence similarity) and Pontibacillus marinus BH030004^T (96.2 %); these three strains formed a robust cluster in the phylogenetic tree. In combination, the phylogenetic, phenotypic and chemotaxonomic data indicate that strain JSM 076056^T represents a novel species of the genus Pontibacillus, for which the name Pontibacillus halophilus sp. nov. is proposed. The type strain is JSM 076056^T (=CCTCC AA 207029^T =DSM 19796^T =KCTC 13190^T).

A supplementary table showing the fatty acid compositions of strain JSM 076056^T and type strains of the genus Pontibacillus is available with the online version of this paper.

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