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Int J Syst Evol Microbiol 59 (2009), 1603-1608; DOI  10.1099/ijs.0.003871-0
© 2009 International Union of Microbiological Societies

Serratia nematodiphila sp. nov., associated symbiotically with the entomopathogenic nematode Heterorhabditidoides chongmingensis (Rhabditida: Rhabditidae)

Chong-Xing Zhang1,2,3,{dagger}, Shou-Yun Yang1,2,3,{dagger}, Ming-Xu Xu1,2,3,{dagger}, Jie Sun1, Huan Liu3, Jing-Rui Liu1, Hui Liu1,2, Fei Kan1, Jing Sun1, Ren Lai1,3 and Ke-Yun Zhang1,2,3

1 College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China
2 Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China
3 Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China

Correspondence
Ke-Yun Zhang
keyunzhang{at}njau.edu.cn
Ren Lai
rlai72{at}njau.edu.cn

A novel red-pigmented, Gram-negative, motile, fluorescent, rod-shaped strain, DZ0503SBS1T, with a single lateral flagellum, was isolated from the intestine of the nematode Heterorhabditidoides chongmingensis. Comparative 16S rRNA gene sequence analysis indicated that the strain is a member of the genus Serratia, sharing highest sequence similarities with Serratia marcescens subsp. sakuensis JCM 11315T (99.8 %), S. marcescens subsp. marcescens DSM 30121T (99.5 %) and Serratia ureilytica LMG 22860T (98.3 %). Similarities between the rpoB gene sequence of strain DZ0503SBS1T and those of S. marcescens subsp. sakuensis JCM 11315T, S. marcescens subsp. marcescens DSM 30121T and S. ureilytica LMG 22860T were 98.0, 97.4 and 98.3 %, respectively. DNA–DNA hybridization values of strain DZ0503SBS1T with S. marcescens subsp. sakuensis JCM 11315T, S. marcescens subsp. marcescens DSM 30121T and S. ureilytica LMG 22860T were 68.2, 65.1 and 53.0 %, respectively. The major isoprenoid quinone of strain DZ0503SBS1T was Q-8 and the predominant fatty acids were C16 : 0 (34.76 %), cyclo-C17 : 0 (20.03 %) and cyclo-C19 : 0{omega}8c (17.24 %). The cyclo-C19 : 0{omega}8c content (17.24 %) was significantly different from those found in S. marcescens subsp. sakuensis JCM 11315T and S. marcescens subsp. marcescens DSM 30121T. Some characteristics of strain DZ0503SBS1T, i.e. fluorescence and its symbiotic association with nematodes, have not been reported previously in any species of the genus Serratia. Phenotypic and biochemical characteristics and molecular data show that strain DZ0503SBS1T represents a novel species, for which the name Serratia nematodiphila sp. nov. is proposed; the type strain is DZ0503SBS1T (=KCTC 22130T =CGMCC 1.6853T).

{dagger}These authors contributed equally to this work.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain DZ0503SBS1T is EU036987.

A neighbour-joining phylogenetic tree based on partial rpoB gene sequences, a fluorescence micrograph showing the location of strain DZ0503SBS1T in the intestine of the nematode Heterorhabditidoides chongmingensis, a transmission electron micrograph of strain DZ0503SBS1T, cellular fatty acid composition data and similarity coefficient values of strain DZ0503SBS1T and related taxa are available with the online version of this paper.






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