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Bowmanella pacifica sp. nov., isolated from a pyrene-degrading consortium

Qiliang Lai^1,, Jun Yuan^1,2,, Baojiang Wang¹, Fengqin Sun¹, Nan Qiao¹, Tianling Zheng² and Zongze Shao¹

¹ Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China
² MOE of Key Lab for Coast and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen 361005, PR China

Correspondence
Zongze Shao
shaozz{at}163.com
Tianling Zheng
wshwzh{at}jingxian.xmu.edu.cn

A taxonomic study was carried out on a strain, designated W3-3A^T, which was isolated from a pyrene-degrading consortium, enriched from sediment of the Pacific Ocean. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain W3-3A^T belonged to the genus Bowmanella, with the highest sequence similarity (99.0 %) with Bowmanella denitrificans BD1^T, whereas sequence similarities with other species were less than 93 %. The nucleotide sequence similarity of both gyrB and rpoD genes of strain W3-3A^T and B. denitrificans BD1^T was 81.1 %. However, the protein sequence similarities of the gyrB and rpoD genes of strain W3-3A^T and B. denitrificans BD1^T were 96.1 % and 91.0 %, respectively. Phylogenetic trees based on these housekeeping genes showed that strain W3-3A^T and B. denitrificans BD1^T formed a distinct lineage in the Gammaproteobacteria. The DNA–DNA hybridization value between strain W3-3A^T and B. denitrificans BD1^T was 43 %. Strain W3-3A^T could also be differentiated from B. denitrificans BD1^T based on the repetitive extragenic palindromic DNA-PCR fingerprint. The G+C content of the chromosomal DNA of strain W3-3A^T was 49 mol%. The combined genotypic and phenotypic data showed that strain W3-3A^T represents a novel species of the genus Bowmanella, for which the name Bowmanella pacifica sp. nov. is proposed, with the type strain W3-3A^T (=CGMCC 1.7086^T=LMG 24568^T=MCCC 1A01018^T).

These authors contributed equally to this work.

The GenBank/EMBL/DDBJ accession numbers for the nucleotide sequences reported in this study are EU440951 (Bowmanella pacifica W3-3A^T, 16S rRNA gene), EU441038 (B. pacifica W3-3A^T, partial gyrB gene), EU441040 (B. pacifica W3-3A^T, partial rpoD gene), EU441037 (Bowmanella denitrificans BD1^T, partial gyrB gene) and EU441039 (B. denitrificans BD1^T, partial rpoD gene).

Transmission electron micrographs of cells of strain W3-3A^T, dendrograms showing the phylogenetic positions of strain W3-3A^T and related species based on gyrB and rpoD gene sequences, and results of rep-PCR and polar lipid analysis of strain W3-3A^T and B. denitrificans BD1^T and a table showing the cellular fatty acid contents of strain W3-3A^T and B. denitrificans BD1^T are available as supplementary material with the online version of this paper.