IJSEM Journal of Bacteriology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Figure
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Google Scholar
Right arrow Articles by Kämpfer, P.
Right arrow Articles by Busse, H.-J.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kämpfer, P.
Right arrow Articles by Busse, H.-J.
Agricola
Right arrow Articles by Kämpfer, P.
Right arrow Articles by Busse, H.-J.
Int J Syst Evol Microbiol 59 (2009), 1545-1549; DOI  10.1099/ijs.0.006841-0
© 2009 International Union of Microbiological Societies

Tessaracoccus lubricantis sp. nov., isolated from a metalworking fluid

Peter Kämpfer1, Nicole Lodders1, Isabel Warfolomeow2 and Hans-Jürgen Busse3

1 Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany
2 Berufsgenossenschaft Metall Nord Süd (BGM), Sachgebiet Biologische Noxen, D-55130 Mainz, Germany
3 Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria

Correspondence
Peter Kämpfer
peter.kaempfer{at}umwelt.uni-giessen.de

A Gram-positive-staining, coccoid-shaped, oxidase-negative, non-spore-forming, non-motile bacterium, strain KSS-17SeT, was isolated from a metalworking fluid. On the basis of its major fatty acid (ai-C15 : 0) and 16S rRNA gene sequence similarity, the strain grouped with Tessaracoccus bendigoensis and Tessaracoccus flavescens, sharing 95.3 and 97.4 % 16S rRNA gene sequence similarity with the respective type strains. Similarities with other established species of the genera Luteococcus, Propioniferax and Granulicoccus were lower than 95.5 %. The quinone system was characterized by the major menaquinone MK-9(H4). In the polar lipid profile, diphosphatidylglycerol, phosphatidylglycerol, an unknown glycolipid and an unknown polar lipid were detected as major compounds. Additionally, three unknown glycolipids and minor amounts of phosphatidylethanolamine, two unknown aminolipids and two unknown polar lipids were detected. Phosphatidylinositol was present only in trace amounts. Predominant polyamines were spermine and spermidine. LL-Diaminopimelic acid was identified as the diagnostic diamino acid in the cell wall. The strain showed clear differences in phenotype (including chemotaxonomic features) from both Tessaracoccus species and members of the other above-mentioned genera. DNA–DNA hybridization between KSS-17SeT and T. bendigoensis Ben-106T and T. flavescens SST-39T yielded similarities of 15.1 and 21.0 %, respectively. It is evident that the organism represents a novel species, for which the name Tessaracoccus lubricantis sp. nov. is proposed. The type strain is KSS-17SeT (=DSM 19926T =CCUG 55516T).

Abbreviations: pNA, p-nitroanilide; pNP, p-nitrophenyl

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain KSS-17SeT is FM178840.

The polar lipid profile of KSS-17SeT after two-dimensional TLC is available as supplementary material with the online version of this paper.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2009 by the International Union of Microbiological Societies.