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Int J Syst Evol Microbiol 59 (2009), 1535-1540; DOI  10.1099/ijs.0.006247-0
© 2009 International Union of Microbiological Societies

Bifidobacterium mongoliense sp. nov., from airag, a traditional fermented mare's milk product from Mongolia

Koichi Watanabe1, Hiroshi Makino1, Masae Sasamoto1, Yuko Kudo1, Junji Fujimoto1 and Shirchin Demberel2

1 Yakult Central Institute for Microbiological Research, 1796 Yaho, Kunitachi, Tokyo 186-8650, Japan
2 Mongolian State University of Agriculture, Zaisan-53, MSUA Ulaanbaatar 210153, Mongolia

Correspondence
Koichi Watanabe
koichi-watanabe{at}yakult.co.jp

Two novel micro-organisms, designated strains YIT 10443T and YIT 10738, were isolated from airag, a traditional fermented mare's milk from Mongolia. The two strains were Gram-positive-staining, non-motile, asporogenous, catalase-negative, facultatively anaerobic rods of various shapes. Comparative analyses of 16S rRNA and ClpC ATPase (clpC) gene sequences and the presence of fructose-6-phosphate phosphoketolase (F6PPK) demonstrated that the novel strains were members of the genus Bifidobacterium. On the basis of 16S rRNA gene sequence similarity, the type strains of Bifidobacterium minimum (96.6 %) and Bifidobacterium psychraerophilum (95.7 %) were the closest neighbours of the novel strains, and DNA–DNA reassociation values with these strains were found to be lower than 15 %. The phenotypic and genotypic features demonstrated that the two strains represent a single, novel Bifidobacterium species, for which the name Bifidobacterium mongoliense sp. nov. is proposed. The type strain is YIT 10443T (=JCM 15461T =DSM 21395T).

Abbreviations: F6PPK, fructose-6-phosphate phosphoketolase; LAB, lactic acid bacteria; RAPD, randomly amplified polymorphic DNA

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains YIT 10443T, YIT 10738, B. minimum YIT 4097T and B. psychraerophilum YIT 11814T are AB433856, AB433857, AB437350 and AB437351, respectively, and those for the clpC gene sequences of strains YIT 10443T, YIT 10738, B. minimum YIT 4097T and B. psychraerophilum YIT 11814T are AB433858–AB433861, respectively.

RAPD-PCR fingerprints and scanning electron micrographs of the novel strains and a maximum-likelihood 16S rRNA gene sequence-based tree are available as supplementary material with the online version of this paper.






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