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Int J Syst Evol Microbiol 59 (2009), 1524-1530; DOI  10.1099/ijs.0.003814-0
© 2009 International Union of Microbiological Societies

Phaselicystis flava gen. nov., sp. nov., an arachidonic acid-containing soil myxobacterium, and the description of Phaselicystidaceae fam. nov.

Ronald O. Garcia1, Hans Reichenbach2, Michael W. Ring1 and Rolf Müller1

1 UdS – Department of Pharmaceutical Biotechnology, Saarland University, D-66041 Saarbrücken, Germany
2 HZI – Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstraße 7, D-38124 Braunschweig, Germany

Correspondence
Rolf Müller
rom{at}mx-uni.saarland.de

A bacterial strain designated SBKo001T was isolated from a forest soil sample from Mt Makiling in Laguna, Philippines. It shows the general characteristics associated with myxobacteria, such as swarming of Gram-negative, rod-shaped vegetative cells, fruiting body formation and bacteriolytic activity. The strain is mesophilic, strictly aerobic and chemoheterotrophic and also exhibits resistance to various antibiotics. Major fatty acids are iso-C15 : 0, C17 : 1 2-OH and C20 : 4 (arachidonic acid). The G+C content of the genomic DNA is 69.2 mol%. A reference strain, NOSO-1 (=DSM 53757), isolated from the Etosha Basin in Namibia, shares nearly the same characteristics with SBKo001T. The identical 16S rRNA gene sequences of the two strains show 94 % identity to strains of the cellulose-degrading Byssovorax and Sorangium species. Phylogenetic analysis reveals a novel branch diverging from the Polyangiaceae, Sorangiineae, Myxococcales. Their uniqueness in morphological growth stages, unusual fatty acid profile, broad-spectrum antibiotic resistance and branch divergence from the Polyangiaceae imply that strains SBKo001T and NOSO-1 not only represent a novel genus and species, proposed here as Phaselicystis flava gen. nov., sp. nov., but also belong to a new family, Phaselicystidaceae fam. nov. The type strain of Phaselicystis flava is SBKo001T (=DSM 21295T =NCCB 100230T).

Abbreviations: PUFA, polyunsaturated fatty acid

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain SBKo001T is EU545827.

Further images of various growth phases of strains SBKo001T and NOSO-1 are available as supplementary material with the online version of this paper.






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