HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Figures and Table Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Google Scholar Articles by Romanenko, L. A. Articles by Mikhailov, V. V. PubMed PubMed Citation Articles by Romanenko, L. A. Articles by Mikhailov, V. V. Agricola Articles by Romanenko, L. A. Articles by Mikhailov, V. V.

Psychrobacter fulvigenes sp. nov., isolated from a marine crustacean from the Sea of Japan

¹ Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch, Russian Academy of Sciences, Prospect 100 Let Vladivostoku 159, 690022 Vladivostok, Russia
² NODAI Culture Collection Center, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan

Correspondence
Lyudmila A. Romanenko
lro{at}piboc.dvo.ru

Two novel Psychrobacter-like bacteria, strains KC 40^T and KC 65, were isolated from a marine crustacean specimen collected from the Sea of Japan, and were characterized by using a polyphasic approach. Strains were selected on the basis of their ability to produce black–brown diffusible pigments on commonly used organic media, which appears to be a unique characteristic of recognized members of the genus Psychrobacter. Phylogenetic analyses based on both 16S rRNA and gyrB gene sequences showed that the novel isolates formed a separate cluster within the genus Psychrobacter. Strains KC 40^T and KC 65 shared highest levels of 16S rRNA gene sequence similarity with Psychrobacter urativorans DSM 14009^T (98.0 %), Psychrobacter pulmonis CCUG 46240^T (97.9 %), Psychrobacter cibarius JG-219^T (97.9 %), Psychrobacter faecalis Iso-46^T (97.8 %), Psychrobacter aquimaris SW-210^T (97.6 %), Psychrobacter namhaensis SW-242^T (97.6 %) and Psychrobacter nivimaris 88/2-7^T (97.6 %). DNA–DNA hybridization experiments revealed 84 % DNA–DNA relatedness between strains KC 40^T and KC 65 but much lower levels of relatedness (7–35 %) between the novel strains and the type strains of recognized Psychrobacter species, confirming their assignment to a single novel species of the genus Psychrobacter. The two novel strains could be distinguished from recognized species of the genus Psychrobacter based on a combination of physiological and biochemical characteristics. On the basis of phenotypic and molecular properties, strains KC 40^T and KC 65 are considered to represent a novel species of the genus Psychrobacter, for which the name Psychrobacter fulvigenes sp. nov. is proposed. The type strain is KC 40^T (=KMM 3954^T=NRIC 0746^T=JCM 15525^T).

Maximum-parsimony phylogenetic trees based on 16S rRNA and gyrB gene sequences showing the positions of strains KC 40^T and KC 65 among species of the genus Psychrobacter, and a table giving the cellular fatty acid contents of strains KC 40^T and KC 65 are available with the online version of this paper.