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Int J Syst Evol Microbiol 59 (2009), 1474-1479; DOI  10.1099/ijs.0.001552-0
© 2009 International Union of Microbiological Societies

Alcanivorax hongdengensis sp. nov., an alkane-degrading bacterium isolated from surface seawater of the straits of Malacca and Singapore, producing a lipopeptide as its biosurfactant

Yehui Wu, Qiliang Lai, Zhongwen Zhou, Nan Qiao, Chenli Liu and Zongze Shao

Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China

Correspondence
Zongze Shao
shaozz{at}163.com

A taxonomic study was carried out on strain A-11-3T, which was isolated from an oil-enriched consortia from the surface seawater of Hong-Deng dock in the Straits of Malacca and Singapore. Cells were aerobic, Gram-negative, non-spore-forming irregular rods. The strain was catalase- and oxidase-negative. It grew on a restricted spectrum of organic compounds, including some organic acids and alkanes. 16S rRNA gene sequence comparisons showed that strain A-11-3T was most closely related to the type strains of Alcanivorax jadensis (96.8 % sequence similarity), Alcanivorax borkumensis (96.8 %), Alcanivorax dieselolei (94.8 %), Alcanivorax venustensis (94.2 %) and Alcanivorax balearicus (94.0 %). The predominant fatty acids were C16 : 0 (31.2 %), C18 : 1{omega}7c (24.8 %), C18 : 0 (9.6 %), C12 : 0 (8.3 %), C16 : 1{omega}7c (8.3 %) and C16 : 0 3-OH (5.1 %). The G+C content of the genomic DNA was 54.7 mol%. Moreover, the strain produced lipopeptides as its surface-active compounds. According to physiological and biochemical tests, DNA–DNA hybridization results and sequence comparisons of the 16S–23S internal transcribed spacer, the gyrB gene and the alkane hydroxylase gene alkB1, strain A-11-3T was affiliated with the genus Alcanivorax but could be readily distinguished from recognized Alcanivorax species. Therefore strain A-11-3T represents a novel species of the genus Alcanivorax for which the name Alcanivorax hongdengensis sp. nov. is proposed. The type strain is A-11-3T (=CGMCC 1.7084T=LMG 24624T=MCCC 1A01496T).

Abbreviations: ITS, internal transcribed spacer

The GenBank/EMBL/DDBJ accession numbers for the nucleotide sequences of strain A-11-3T are EU438901 (16S rRNA gene), EU438896 (large ITS), EU438897 (small ITS), EU438899 (partial gyrB gene) and EU438898 (partial alkB1 gene).

Transmission electron micrographs of cells of strain A-11-3T, dendrograms showing the phylogenetic position of strain A-11-3T based on ITS, gyrB and alkB sequences and a table detailing the cellular fatty acid profiles of strain A-11-3T and recognized species of the genus Alcanivorax are available as supplementary material in IJSEM Online.






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Copyright © 2009 by the International Union of Microbiological Societies.