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Int J Syst Evol Microbiol 59 (2009), 1416-1420; DOI  10.1099/ijs.0.006189-0
© 2009 International Union of Microbiological Societies

Pseudomonas cuatrocienegasensis sp. nov., isolated from an evaporating lagoon in the Cuatro Ciénegas valley in Coahuila, Mexico

Ana E. Escalante1, Jesús Caballero-Mellado2, Lourdes Martínez-Aguilar2, Alejandra Rodríguez-Verdugo1, Andrea González-González1, Jeiry Toribio-Jiménez3 and Valeria Souza1

1 Departamento de Ecología Evolutiva, Instituto de Ecología, Universidad Nacional Autónoma de México, Apartado Postal 70-275, México DF 04510, Mexico
2 Programa de Ecología Genómica, Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Apartado Postal 565-A, Cuernavaca, Morelos, Mexico
3 Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Apartado Postal 70-275, DF, Mexico

Correspondence
Valeria Souza
souza{at}servidor.unam.mx

Nine Gram-negative, rod-shaped, non-spore-forming isolates with identical or very similar repetitive-sequence-based PCR profiles were recovered from an evaporative lagoon in Mexico. Two strains, designated 1NT and 3N, had virtually identical 16S rRNA gene sequences and, on the basis of these sequences, were identified as members of the genus Pseudomonas, with Pseudomonas peli R-20805T as the closest relative. All nine isolates had practically identical whole-cell protein profiles. The major fatty acids [C16 : 0, C18 : 1{omega}7c and summed feature a (C16 : 1{omega}7 and/or C16 : 1{omega}6c)] of strains 1NT and 3N supported their affiliation with the genus Pseudomonas. The DNA–DNA reassociation values with respect to P. peli LMG 23201T and other closely related Pseudomonas species were <15 %. Physiological and biochemical tests allowed phenotypic differentiation of the strains analysed, including strain 1NT, from the five phylogenetically closest Pseudomonas species. On the basis of the data obtained by using this polyphasic taxonomic approach, the nine strains represent a novel species, for which the name Pseudomonas cuatrocienegasensis sp. nov. is proposed. The type strain is 1NT (=LMG 24676T=CIP 109853T).

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA sequences of strains 1NT and 3N are EU791281 and EU791282, respectively.

A figure showing a pulsed-field electrophoresis gel of novel strains described in this work and type and reference strains of known Pseudomonas species is available with the online version of this paper.






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