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Description of Acinetobacter venetianus ex Di Cello et al. 1997 sp. nov.

¹ Laboratory Bacteriology Research (LBR), Department of Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, 9000 Gent, Belgium
² Centre of Epidemiology and Microbiology, National Institute of Public Health, 100 42 Prague, Czech Republic
³ Department of Infectious Diseases, Leiden University Medical Center C5-P, PO Box 9600, 2300 RC Leiden, The Netherlands
⁴ Department of Medical Microbiology, Malmö University Hospital, University of Lund, S-205 02 Malmö, Sweden
⁵ Kerry Group, Kerry 1327 AH Almere, The Netherlands
⁶ Department of Evolutionary Biology, University of Firenze, I-50125 Firenze, Italy

Correspondence
Mario Vaneechoutte
Mario.Vaneechoutte{at}UGent.be

The name ‘Acinetobacter venetianus’ has been used previously to designate three marine hydrocarbon-degrading Acinetobacter strains, of which strain RAG-1 (=ATCC 31012) has industrial applications for the production of the bioemulsifier emulsan. However, to date, the name of this taxon has not been validly published. In this study, five strains were examined to corroborate the delineation of this taxon by means of phenotypic characterization, DNA–DNA hybridization, selective restriction fragment amplification (AFLP), amplified rDNA restriction analysis (ARDRA), rpoB gene sequence analysis and tRNA intergenic spacer length polymorphism analysis (tDNA-PCR) and to emend the description of ‘Acinetobacter venetianus’ (ex Di Cello et al. 1997). AFLP analysis showed that the five strains formed a tight cluster at 56.8±5.0 % genomic relatedness that was separated from strains of other haemolytic species of the genus Acinetobacter and from the type and reference strains of other Acinetobacter species at 27 % relatedness, indicating the distinctiveness of the novel strains. The strains were haemolytic and able to grow on citrate (Simmons), L-histidine and malonate. The strains did not oxidize D-glucose or utilize DL-lactate or L-aspartate. The G+C contents of strains RAG-1 and of VE-C3 were 43.9 % and 43.6 mol%, respectively. The novel strains could be recognized by a characteristic ARDRA pattern (CfoI 1, AluI 3, MboI 2, RsaI 2, MspI 3). The consensus tDNA-PCR pattern for the five strains consisted of amplified fragments of 87.9, 100.2, 134.6 and 248.5 bp and was indistinguishable from that of strains of Acinetobacter genomic species 14BJ. The five strains represent a novel species for which the name Acinetobacter venetianus sp. nov. is proposed. The type strain is RAG-1^T (=ATCC 31012^T=CCUG 45561^T=LMG 19082^T=LUH 3904^T=NIPH 1925^T).

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences are AM909651 and EU258608–EU258610. The accession numbers for the rpoB partial gene sequences are EU477136 and EU496378–EU496381.

A supplementary figure showing the growth of different Acinetobacter strains after 72 h in mineral medium containing carbon sources of variable chain lengths and a supplementary table showing the P-values of the differences in delta yield over the range of carbon sources used between various Acinetobacter strains and strain RAG-1 are available with the online version of this paper.

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