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Int J Syst Evol Microbiol 59 (2009), 1371-1375; DOI  10.1099/ijs.0.007799-0
© 2009 International Union of Microbiological Societies

Phylogenetic analysis of Mycobacterium aurum and Mycobacterium neoaurum with redescription of M. aurum culture collection strains

Keith E. Simmon1, Yan Yi Low2, Barbara A. Brown-Elliott3, Richard J. Wallace, Jr3 and Cathy A. Petti1,4

1 Associated Regional and University Pathologists (ARUP) Institute for Clinical and Experimental Pathology, Salt Lake City, UT, USA
2 Associated Regional and University Pathologists (ARUP) Laboratories, Salt Lake City, UT, USA
3 University of Texas Health Science Center, Department of Microbiology, Tyler, TX, USA
4 Departments of Medicine and Pathology, University of Utah School of Medicine, Salt Lake City, UT, USA

Correspondence
Keith E. Simmon
keith.simmon{at}aruplab.com

We examined American Type Culture Collection (ATCC) strains of Mycobacterium aurum and Mycobacterium neoaurum by using multilocus DNA target sequencing. Apart from the type strain, all 10 ATCC M. aurum strains examined were classified incorrectly, with most being reclassified as belonging to the M. neoaurum–‘Mycobacterium lacticola’ relatedness group. All four M. neoaurum strains were tightly clustered, but heterogeneity was observed within the cluster. As a result of the incorrect annotation of the M. aurum strains, two commonly used methods of identification are compromised and two case reports implicating M. aurum as a human pathogen are probably incorrect, with the isolates probably belonging to the M. neoaurum–‘M. lacticola’ relatedness group. These findings together with a review of isolates identified at two large reference laboratories suggest that M. aurum is not a clinically significant isolate.

Abbreviations: ARUP, Associated Regional and University Pathologists; ATCC, American Type Culture Collection; PRA, PCR-restriction fragment length polymorphism analysis; UTHSCT, University of Texas Health Science Center

The GenBank/EMBL/DDBJ accession numbers for the sequences determined in this study are given in Supplementary Table S1 in IJSEM Online.

Neighbour-joining trees based on rpoB and hsp65 gene sequences and a table of the ATCC strains used and GenBank accession numbers for the genes investigated in this study are available as supplementary material with the online version of this paper.






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