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Int J Syst Evol Microbiol 59 (2009), 1348-1352; DOI  10.1099/ijs.0.006643-0
© 2009 International Union of Microbiological Societies

Description of Leifsonia kafniensis sp. nov. and Leifsonia antarctica sp. nov.

Pavan Kumar Pindi{dagger}, K. Hara Kishore{dagger}, G. S. N. Reddy and S. Shivaji

Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India

Correspondence
S. Shivaji
shivas{at}ccmb.res.in

Strains KFC-22T and SPC-20T are yellow-pigmented, Gram-positive, aerobic, non-motile, rod-shaped bacteria that were isolated from a soil sample near the Kafni glacier in the Himalayan mountain ranges in India, and from a spade core sediment sample from the Antarctic Ocean at Larsemann Hill, respectively. In both cases, the cell-wall peptidoglycan contained 2,4-diaminobutyric acid as the diamino acid, anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0 were the predominant fatty acids and MK-11 was the major isoprenoid quinone in the cell membrane. On the basis of the above-mentioned characteristics, both strains can be assigned to the genus Leifsonia. The strains share 16S rRNA gene sequence similarity of 97.7 % and DNA relatedness of only 10 %, indicating that they represent different species. A BLAST analysis indicated that Leifsonia pindariensis PON10T was the closest phylogenetic neighbour of strains SPC-20T and KFC-22T, showing 16S rRNA gene sequence similarities of 97.3 and 97.7 %, respectively. However, at the whole-genome level, strains KFC-22T and SPC-20T shared 42 and 11 % DNA–DNA relatedness, respectively, with L. pindariensis PON10T. In addition, both strains exhibited several phenotypic differences with respect to L. pindariensis PON10T. Thus, on the basis of the differences that the two strains exhibited with respect to L. pindariensis, both were identified as representing novel species of the genus Leifsonia, for which the names Leifsonia kafniensis sp. nov. (type strain KFC-22T =NCCB 100216T =LMG 24362T) and Leifsonia antarctica sp. nov. (type strain SPC-20T =NCCB 100227T =LMG 24541T) are proposed.

{dagger}These authors contributed equally to this work.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains KFC-22T and SPC-20T are AM889135 and AM931710, respectively.

16S rRNA gene sequence similarities of strains KFC-22T and SPC-20T with respect to closely related strains are available as supplementary material with the online version of this paper.






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