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Pseudomonas cedrina subsp. fulgida subsp. nov., a fluorescent bacterium isolated from the phyllosphere of grasses; emended description of Pseudomonas cedrina and description of Pseudomonas cedrina subsp. cedrina subsp. nov.

¹ Leibniz-Centre for Agricultural Landscape Research (ZALF), Institute of Landscape Matter Dynamics, Eberswalder Str. 84, D-15374 Müncheberg, Germany
² DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, D-38124 Braunschweig, Germany
³ Département Microorganismes, Génomes, Environnement, Université Louis-Pasteur-CNRS, UMR 7156, F-67000 Strasbourg, France

Correspondence
Undine Behrendt
ubehrendt{at}zalf.de

The taxonomic position of a group of four strains, isolated from the phyllosphere of grasses, within the species Pseudomonas cedrina was investigated. The isolates formed a separate cluster through ribotyping and MALDI-TOF MS, which could be clearly differentiated from the type strain of P. cedrina. The differences found between the patterns of the type strain of P. cedrina and the novel isolates were more distinct than those between the type strain and recognized species of the genus Pseudomonas, which were phylogenetically related by 16S rRNA gene sequence analysis. Physiological characterization also revealed significant differences between the novel grass isolates and the type strain of P. cedrina. Siderotyping of the pyoverdines revealed identical pyoverdine-isoelectrofocusing patterns for the novel isolates and the type strain of P. cedrina. However, pyoverdine-mediated ⁵⁹Fe cross uptake studies indicated differences in the siderotype. In contrast, phylogenetic analysis based on 16S rRNA gene sequence analysis and DNA–DNA hybridization studies (reassociation value 76.4 %) supported the affiliation of the novel isolates to the species P. cedrina. As a consequence of these observations, the splitting of the species P. cedrina into two novel subspecies Pseudomonas cedrina subsp. cedrina subsp. nov. (type strain CFML 96-198^T=CIP 105541^T=DSM 17516^T) and Pseudomonas cedrina subsp. fulgida subsp. nov. (type strain P 515/12^T=DSM 14938^T=LMG 21467^T) is proposed.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain P 515/12^T is AJ492830.

A supplementary table detailing the differential physiological characteristics of P. cedrina subsp. cedrina subsp. nov., P. cedrina subsp. fulgida subsp. nov. and related species and a supplementary figure showing the Riboprint patterns of EcoRI-restricted DNA from the novel isolates and related strains are available with the online version of this paper.