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Kocuria halotolerans sp. nov., an actinobacterium isolated from a saline soil in China

Shu-Kun Tang^1,, Yun Wang^2,, Kai Lou², Pei-Hong Mao³, Li-Hua Xu¹, Cheng-Lin Jiang¹, Chang-Jin Kim⁴ and Wen-Jun Li^1,5

¹ The Key Laboratory for Microbial Resources of the Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China
² Xinjiang Institute of Microbiology, Xinjiang Academy of Agricultural Science, Urumqi, Xinjiang 830091, PR China
³ Institute of Ion Beam Biotechnology, College of Physics Science and Technology, Xinjiang University, Urumqi, Xinjiang 830008, PR China
⁴ Functional Metabolite Research Center KRIBB, 52 Eoeun-dong, Yuseong gu, Daejeon 305-806, Republic of Korea
⁵ Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China

Correspondence
Wen-Jun Li
wjli{at}ynu.edu.cn
or
liact{at}hotmail.com

A Gram-positive actinobacterium, designated strain YIM 90716^T, was isolated from a saline soil sample collected from Ganjiahu Suosuo Forest National Nature Reserve in Xinjiang Province, north-west China. The new isolate contained lysine, glutamic acid and alanine with peptidoglycan type Lys–Ala₃ (variation A3). The major phospholipids were phosphatidylglycerol and diphosphatidylglycerol. The predominant menaqinone was MK-7(H₂). The major fatty acids were anteiso-C_{15 : 0}, iso-C_{16 : 0} and anteiso-C_{17 : 0}. The DNA G+C content of strain YIM 90716^T was 68.0 mol%. Chemotaxonomic properties supported the affiliation of strain YIM 90716^T to the genus Kocuria. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the organism was related most closely to Kocuria kristinae DSM 20032^T (96.8 % similarity) and showed lower levels of 16S rRNA gene similarity (<96.5 %) with the type strains of other species of the genus Kocuria. The results of fatty acid analysis and physiological and biochemical tests allowed the genotypic and phenotypic differentiation of strain YIM 90716^T from its closest relatives. On the basis of data from the present polyphasic study, strain YIM 90716^T is considered to represent a novel species of the genus Kocuria, for which the name Kocuria halotolerans sp. nov. is proposed. The type strain is YIM 90716^T (=DSM 18442^T=KCTC 19172^T=CCTCC AB 206069^T).

These authors contributed equally to this work.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain YIM 90716^T is DQ979377.

A chromatogram of amino acids in purified cell-wall and whole-cell hydrolysates of strain YIM 90716^T and a table detailing the cellular fatty acid compositions of strain YIM 90716^T and Kocuria kristinae DSM 20032^T are available as supplementary material with the online version of this paper.