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Nautilia abyssi sp. nov., a thermophilic, chemolithoautotrophic, sulfur-reducing bacterium isolated from an East Pacific Rise hydrothermal vent

¹ UMR6197, Laboratoire de Microbiologie des Environnements Extrêmes, IUEM, Technopôle Brest-Iroise, F-29280 Plouzané, France
² Laboratoire Environnement Profond, Ifremer EEP/LEP, BP 70, F-29280 Plouzané, France

Correspondence
Karine Alain
Karine.Alain{at}univ-brest.fr

A novel strictly anaerobic, thermophilic, sulfur-reducing bacterium, designated PH1209^T, was isolated from an East Pacific Rise hydrothermal vent (1 ° N) sample and studied using a polyphasic taxonomic approach. Cells were Gram-negative, motile rods (approx. 1.60x0.40 µm) with a single polar flagellum. Strain PH1209^T grew at temperatures between 33 and 65 °C (optimum 60 °C), from pH 5.0 to 8.0 (optimum 6.0–6.5), and between 2 and 4 % (w/v) NaCl (optimum 3 %). Cells grew chemolithoautotrophically with H₂ as an energy source, S⁰ as an electron acceptor and CO₂ as a carbon source. Strain PH1209^T was also able to use peptone and yeast extract as carbon sources. The G+C content of the genomic DNA was 35 mol%. Phylogenetic analyses based on 16S rRNA gene sequencing showed that strain PH1209^T fell within the order Nautiliales, in the class Epsilonproteobacteria. Comparative 16S rRNA gene sequence analysis indicated that strain PH1209^T belonged to the genus Nautilia and shared 97.2 and 98.7 % 16S rRNA gene sequence identity, respectively, with the type strains of Nautilia lithotrophica and Nautilia profundicola. It is proposed, from the polyphasic evidence, that the strain represents a novel species, Nautilia abyssi sp. nov.; the type strain is PH1209^T (=DSM 21157^T=JCM 15390^T).

Scanning electron micrographs of cells of strain PH1209^T (Fig. S1) and a graph showing the maximum growth rate of strain PH1209^T at various temperatures, pH and NaCl concentrations (Fig. S2) are available with the online version of this paper.