HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Nishiyama, T. Articles by Ueki, K. Search for Related Content PubMed PubMed Citation Articles by Nishiyama, T. Articles by Ueki, K. Agricola Articles by Nishiyama, T. Articles by Ueki, K.

Clostridium sufflavum sp. nov., isolated from a methanogenic reactor treating cattle waste

Faculty of Agriculture, Yamagata University, Wakaba-machi 1-23, Tsuruoka, Yamagata 997-8555, Japan

Correspondence
Atsuko Ueki
uatsuko{at}tds1.tr.yamagata-u.ac.jp

A strictly anaerobic, mesophilic, cellulolytic bacterial strain, designated CDT-1^T, was isolated from rice-straw residue from a methanogenic reactor treating waste from cattle farms. The isolation was performed using enrichment culture with filter paper as a substrate. Cells stained Gram-negative, but reacted Gram-positively in the KOH test. Cells were slightly curved rods and were motile by means of peritrichous flagella. The strain produced yellow pigment when grown on filter-paper fragments. Although spore formation was not confirmed microscopically, thermotolerant cells were produced when the strain was grown on filter paper. The optimum temperature for growth was 33 °C and the optimum pH was 7.4. Oxidase, catalase and nitrate-reducing activities were absent. The strain utilized xylose, fructose, glucose, cellobiose, xylooligosaccharide, cellulose (filter-paper fragments and ball-milled filter paper) and xylan. The major fermentation products were acetate, ethanol, H₂ and CO₂. The major cellular fatty acids were iso-C_{15 : 0}, iso-C_{14 : 0} and C_{16 : 0} DMA. The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. The genomic DNA G+C content was 40.7 mol%. On the basis of 16S rRNA gene sequence similarities, strain CDT-1^T could be placed in cluster III of the genus Clostridium, being closely related to type strains of Clostridium hungatei (96.6 % sequence similarity), Clostridium termitidis (96.2 %) and Clostridium papyrosolvens (96.1 %). On the basis of the cellular, physiological and phylogenetic differences between CDT-1^T and its close relatives, this strain represents a novel species of the genus Clostridium, for which the name Clostridium sufflavum sp. nov. is proposed. The type strain is CDT-1^T (=JCM 14807^T=DSM 19573^T).

This article has been cited by other articles:

				T. Nishiyama, A. Ueki, N. Kaku, K. Watanabe, and K. Ueki Bacteroides graminisolvens sp. nov., a xylanolytic anaerobe isolated from a methanogenic reactor treating cattle waste Int J Syst Evol Microbiol, August 1, 2009; 59(8): 1901 - 1907. [Abstract] [Full Text] [PDF]