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Pyramidobacter piscolens gen. nov., sp. nov., a member of the phylum ‘Synergistetes’ isolated from the human oral cavity

¹ King's College London Dental Institute, Infection Research Group, London SE1 9RT, UK
² Department of Molecular Genetics, The Forsyth Institute, Boston, MA 02115, USA
³ Division of Biomedical Sciences, School of Applied Sciences, Northumbria University, Newcastle upon Tyne NE1 8ST, UK

Correspondence
W. G. Wade
william.wade{at}kcl.ac.uk

Four strains of anaerobic, Gram-negative bacilli isolated from the human oral cavity were subjected to a comprehensive range of phenotypic and genotypic tests and were found to comprise a homogeneous group distinct from any species with validly published names. 16S rRNA and 23S rRNA gene sequence analyses and DNA–DNA reassociation data revealed that the strains constituted a novel group within the phylum ‘Synergistetes’ and were most closely related to Jonquetella anthropi. Two libraries of randomly cloned DNA were prepared from strain W5455^T and were sequenced to provide a genome survey as a resource for metagenomic studies. A new genus and novel species, Pyramidobacter piscolens gen. nov., sp. nov., is proposed to accommodate these strains. The genus Pyramidobacter comprises strains that are anaerobic, non-motile, asaccharolytic bacilli that produce acetic and isovaleric acids and minor to trace amounts of propionic, isobutyric, succinic and phenylacetic acids as end products of metabolism. P. piscolens gen. nov., sp. nov. produced hydrogen sulphide but was otherwise largely biochemically unreactive. Growth was stimulated by the addition of glycine to broth media. The G+C content of the DNA of the type strain was 59 mol%. The type strain of Pyramidobacter piscolens sp. nov. is W5455^T (=DSM 21147^T=CCUG 55836^T).

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains W5455^T, AHN 11662 and P4G_18 are EU309492, EU309493 and AY207056, respectively.

Tables showing the 16S–23S rRNA gene intervening sequences and 23S rRNA primers, and fatty acid methyl ester profiles of strains of Pyramidobacter piscolens gen. nov., sp. nov., Jonquetella anthropi and Dethiosulfovibrio peptidovorans and static annotations of partial and full-length open reading frames in genomic libraries 1 and 2 are available as supplementary material with the online version of this paper.

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				S. R. Vartoukian, R. M. Palmer, and W. G. Wade Diversity and Morphology of Members of the Phylum "Synergistetes" in Periodontal Health and Disease Appl. Envir. Microbiol., June 1, 2009; 75(11): 3777 - 3786. [Abstract] [Full Text] [PDF]