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Desulfovibrio marrakechensis sp. nov., a 1,4-tyrosol-oxidizing, sulfate-reducing bacterium isolated from olive mill wastewater

¹ Anaerobic Microbiology Team (E02B26), Sciences and Techniques Faculty, Cadi Ayyad University, PO Box 549, 40 000 Marrakech, Morocco
² DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7B, D-38124 Braunschweig, Germany
³ REQUIMTE/CQFB, Departamento de Química, FCT/UNL, 2829-516 Caparica, Portugal
⁴ Unidade de Investigação em Biodiversidade e Desenvolvimento, Universidade Lusófona de Humanidades e Tecnologias, Campo Grande 376, 1749-024 Lisboa, Portugal
⁵ School of Science and Engineering, Al Akhawayn University, PO Box 1846, Ifrane, Morocco
⁶ IRD, UMR180 LMBEC, Université de Provence et de La Méditerranée, ESIL, Case 925, 163 Avenue de Luminy, F-13288 Marseille, France

Correspondence
Abdel-Illah Qatibi
qatibi{at}fstg-marrakech.ac.ma
or
qatibi{at}yahoo.fr

A novel mesophilic sulfate-reducing bacterium, EMSSDQ₄^T, was isolated from olive mill wastewater in the semi-arid region of Morocco (Marrakech). Cells were Gram-negative, catalase-positive, straight rods that were non-motile and non-spore-forming and contained cytochrome c₃ and desulfoviridin. The DNA G+C content was 65.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate was a member of the genus Desulfovibrio with Desulfovibrio carbinoliphilus D41^T, Desulfovibrio alcoholivorans SPSN^T, Desulfovibrio fructosivorans JJ^T and Desulfovibrio carbinolicus EDK82^T as the most closely related strains with validly published names. In addition to the classical substrates used by Desulfovibrio species, the isolate oxidized 1,4-tyrosol, one of the most abundant phenolic compounds occurring in olive mill wastewater, to 4-hydroxyphenylacetate without ring cleavage. D. alcoholivorans SPSN^T was also found to carry out this reaction. Under air, strain EMSSDQ₄^T exhibited limited growth on lactate and yeast extract in the absence of sulfate. On the basis of genotypic and phenotypic characteristics, it is proposed that the isolate represents a novel species, Desulfovibrio marrakechensis sp. nov. The type strain is EMSSDQ₄^T (=DSM 19337^T =ATCC BAA-1562^T).

The authors dedicate this paper to the memory of Professor Norbert Pfennig (1925–2008).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain EMSSDQ₄^T is AM947130.

Photomicrographs of cells of strain EMSSDQ₄^T and growth curves of strain EMSSDQ₄^T and related strains cultivated in air in the absence of sulfate are available as supplementary material with the online version of this paper.