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Oceanicola pacificus sp. nov., isolated from a deep-sea pyrene-degrading consortium

Jun Yuan^1,, Qiliang Lai^2,, Baojiang Wang², Fengqin Sun², Xiupian Liu², Yaping Du², Guangyu Li², Li Gu², Tianling Zheng¹ and Zongze Shao²

¹ School of Life Sciences, MOE of Key Lab for Subtropical Wetland Ecosystem, Xiamen University, Xiamen 361005, PR China
² Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China

Correspondence
Tianling Zheng
wshwzh{at}xmu.edu.cn
Zongze Shao
shaozz{at}163.com

A taxonomic study was carried out on a Gram-negative, rod-shaped, non-motile, halophilic bacterium, designated strain W11-2B^T, which was isolated from a pyrene-degrading consortium that was enriched from sediment from the Pacific Ocean. Growth was observed at salinities of 0.5–10 % and at temperatures of 10–41 °C. Strain W11-2B^T was unable to degrade Tween 80 or gelatin. 16S rRNA gene sequence comparisons showed that strain W11-2B^T was related most closely to Oceanicola nanhaiensis SS011B1-20^T (95.8 % similarity) and Oceanicola batsensis HTCC2597^T (95.7 %); levels of 16S rRNA gene sequence similarity between strain W11-2B^T and the type strains of other species tested were below 95.2 %. The dominant fatty acids of strain W11-2B^T were C_{18 : 1}7c (32.1 % of the total), C_{19 : 0} cyclo (20.9 %), C_{18 : 1}7c 11-methyl (19.5 %), C_{18 : 0} (7.3 %), C_{17 : 0} (6.6 %) and C_{16 : 0} (3.8 %). The G+C content of the chromosomal DNA was 64.6 mol%. The above data were in good agreement with those of members of the genus Oceanicola. Based on morphology, physiology, fatty acid composition and 16S rRNA gene sequence data, strain W11-2B^T is considered to represent a novel species of the genus Oceanicola, for which the name Oceanicola pacificus sp. nov. is proposed. The type strain is W11-2B^T (=CCTCC AB 208224^T=LMG 24619^T=MCCC 1A01034^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain W11-2B^T is DQ659449.

A transmission electron micrograph of cells of strain W11-2B^T and a table detailing the cellular fatty acid content of strain W11-2B^T are available as supplementary material with the online version of this paper.