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Int J Syst Evol Microbiol 59 (2009), 1112-1115; DOI  10.1099/ijs.0.006379-0
© 2009 International Union of Microbiological Societies

Corynebacterium lubricantis sp. nov., isolated from a coolant lubricant

Peter Kämpfer1, Nicole Lodders1, Isabel Warfolomeow2, Enevold Falsen3 and Hans-Jürgen Busse4

1 Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany
2 Berufsgenossenschaft Metall Nord Süd – BGM, D-55130 Mainz, Germany
3 Culture Collection University Göteborg, Department of Clinical Bacteriology, S-41346 Göteborg, Sweden
4 Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria

Correspondence
Peter Kämpfer
peter.kaempfer{at}umwelt.uni-giessen.de

Three Gram-positive, rod-shaped, oxidase-negative, non-spore-forming, non-motile bacteria (KSS-3SeT, KSS-4Se and KSS-10Se) were isolated from a coolant lubricant. 16S rRNA gene sequence analyses revealed almost identical sequences, with only a few (<10 positions) differences for these three isolates. Comparisons showed the highest similarities to Corynebacterium pilosum NCTC 11862T (97.6 % similarity with strain KSS-3SeT). Similarities with other established Corynebacterium species were lower than 97.0 %. Chemotaxonomic data studied for strain KSS-3SeT [polar lipids – major compounds phosphatidylglycerol and an unknown glycolipid, moderate amounts of phosphatidylinositol and diphosphatidylglycerol; polyamines (small amounts) – major compounds spermidine and spermine; quinones – significant amounts of menaquinones MK-9(H2), MK-8(H2) and MK-7(H2); and major fatty acids – tuberculostearic acid (10-methyl C18 : 0), C16 : 0 and C18 : 1{omega}9c] were congruent with those reported for Corynebacterium. The strain showed differences in phenotype from C. pilosum. DNA–DNA hybridizations between KSS-3SeT and C. pilosum DSM 20521T yielded a relatedness of 22.9 % (20.4 % in the reciprocal assay). From these results, it is evident that the organisms represent a novel species, for which the name Corynebacterium lubricantis sp. nov. is proposed (type strain KSS-3SeT =DSM 45231T =CCUG 56567T =CCM 7546T).

Abbreviations: pNA, p-nitroanilide; pNP, p-nitrophenyl

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains KSS-3SeT, KSS-4Se and KSS-10Se are FM173119–FM173121, respectively.

An extended neighbour-joining tree and a comparison of biochemical characteristics of the novel species and related members of Corynebacterium are available as supplementary material with the online version of this paper.






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