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Int J Syst Evol Microbiol 59 (2009), 1108-1111; DOI  10.1099/ijs.0.000117-0
© 2009 International Union of Microbiological Societies

Providencia sneebia sp. nov. and Providencia burhodogranariea sp. nov., isolated from wild Drosophila melanogaster

Punita Juneja and Brian P. Lazzaro

Department of Entomology, Cornell University, Ithaca, NY 14853, USA

Correspondence
Brian P. Lazzaro
bl89{at}cornell.edu

Multiple isolates of the genus Providencia were obtained from the haemolymph of wild-caught Drosophila melanogaster fruit flies. Sixteen isolates were distinguished from the six previously described species based on 16S rRNA gene sequences. These isolates belonged to two distinct groups, which we propose each comprise previously undescribed species. Two isolates, designated AT and BT, were characterized by DNA sequences of the fusA, lepA, leuS, gyrB and ileS housekeeping genes, whole-genome DNA–DNA hybridizations with their nearest relatives and utilization of substrates for metabolism. The closest phylogenetic relatives of strain AT are strain BT (86.9 % identity for the housekeeping genes) and Providencia stuartii DSM 4539T (86.0 % identity). The closest phylogenetic relatives of strain BT are strain AT (86.9 % identity) and P. stuartii DSM 4539T (86.6 % identity). The type strains of described species in this genus shared between 84.1 and 90.1 % identity for these sequences. DNA–DNA hybridization between the strain pairs AT–BT, AT–P. stuartii DSM 4539T and BT–P. stuartii DSM 4539T all resulted in less than 25 % relatedness. In addition, patterns of utilization of amygdalin, arbutin, aesculin, salicin, D-sorbitol, trehalose, inositol, D-adonitol and D-galactose distinguish strains AT and BT from other members of this genus. Strains AT and BT therefore represent novel species, for which the names Providencia sneebia sp. nov. (type strain AT =DSM 19967T =ATCC BAA-1589T) and Providencia burhodogranariea sp. nov. (type strain BT =DSM 19968T =ATCC BAA-1590T) are proposed.

The GenBank/EMBL/DDBJ accession numbers for the sequences of the 16S rRNA, ileS, gyrB, fusA, lepA and leuS genes of the type strains of six described Providencia species and the novel strains are EU587018–EU587118, as detailed in Supplementary Table S2.

Details of primers used for PCR amplification of housekeeping genes and sequence accession numbers and a 16S rRNA gene sequence-based neighbour-joining tree are available as supplementary material with the online version of this paper.






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