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Micrococcus endophyticus sp. nov., isolated from surface-sterilized Aquilaria sinensis roots

Hua-Hong Chen^1,2,, Guo-Zhen Zhao^1,, Dong-Jin Park³, Yu-Qin Zhang^1,4, Li-Hua Xu¹, Jae-Chan Lee³, Chang-Jin Kim³ and Wen-Jun Li¹

¹ The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China
² Department of Chemistry and Biology, Chuxiong Normal University, Chuxiong, Yunnan 675000, PR China
³ Functional Metabolite Research Center (KRIBB), 52 Eoeun-dong, Yuseong gu, Daejeon 305-806, Republic of Korea
⁴ Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China

Correspondence
Wen-Jun Li
wjli{at}ynu.edu.cn
or
liact{at}hotmail.com

A Gram-positive bacterial strain, designated YIM 56238^T, was isolated from plant roots (Aquilaria sinensis), and characterized by using a polyphasic approach. Strain YIM 56238^T grew optimally at pH 7.0–8.0 and at 28 °C. Analysis of the 16S rRNA gene sequence of strain YIM 56238^T indicated that it belongs to the genus Micrococcus. Chemotaxonomic data strongly supported the classification of this strain within the genus Micrococcus: the cell-wall peptidoglycan contained lysine, glutamic acid, alanine and glycine; the predominant menaquinones were MK-8(H₂) (63.6 %) and MK-7(H₂) (21.1 %); the phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown ninhydrin-negative phospholipid; and the major cellular fatty acids were iso-C_{15 : 0} (30.95 %) and anteiso-C_{15 : 0} (53.75 %). The G+C content of the genomic DNA was 72.9 mol%. A number of physiological features were found that clearly distinguished strain YIM 56238^T from recognized species of the genus Micrococcus. DNA–DNA hybridization studies suggested that the novel strain represents a separate genomic species. On the basis of the data, therefore, strain YIM 56238^T represents a novel species of the genus Micrococcus, for which the name Micrococcus endophyticus sp. nov. is proposed. The type strain is YIM 56238^T (=DSM 17945^T=KCTC 19156^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain YIM 56238^T is EU005372.

A scanning electron micrograph of cells of strain YIM 56238^T grown on TSA medium is available as a supplementary figure with the online version of this paper.

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