IJSEM Applied and Environmental Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Figure
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Doerfert, S. N.
Right arrow Articles by Ferry, J. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Doerfert, S. N.
Right arrow Articles by Ferry, J. G.
Agricola
Right arrow Articles by Doerfert, S. N.
Right arrow Articles by Ferry, J. G.
Int J Syst Evol Microbiol 59 (2009), 1064-1069; DOI  10.1099/ijs.0.003772-0
© 2009 International Union of Microbiological Societies

Methanolobus zinderi sp. nov., a methylotrophic methanogen isolated from a deep subsurface coal seam

Sebastian N. Doerfert1,{dagger}, Matthew Reichlen1,{dagger}, Prabha Iyer2, Mingyu Wang1 and James G. Ferry1

1 Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802, USA
2 J. Craig Venter Institute, Rockville, MD 20850, USA

Correspondence
James G. Ferry
jgf3{at}psu.edu

A methanogenic organism from the domain Archaea (SD1T) was isolated from saline water released from a coal seam located 926 m below the surface via a methane-producing well near Monroe, Louisiana, USA. Growth and methanogenesis were supported with methanol, monomethylamine, dimethylamine or trimethylamine, but not with dimethylsulfide, formate, acetate or H2/CO2. Cells grew in high-salt minimal medium but growth was stimulated with yeast extract or tryptone. Cells were single, non-motile, irregular coccoids 0.5–1.0 µm in diameter and the cell wall contained protein. Conditions for the maximum rate of growth were 40–50 °C, 0.2–0.6 M NaCl, 100–≥200 mM MgCl2, and pH 7.0–8.0. The G+C content of the genomic DNA was 42±1mol %. A comparison of 16S rRNA gene sequences indicated that strain SD1T was most closely related to Methanolobus oregonensis DSM 5435T with 96 % gene sequence similarity. It is proposed that strain SD1T represents a novel species, Methanolobus zinderi sp. nov. The type strain is SD1T (=ATCC BAA-1601T=DSM 21339T).

Abbreviations: CBM, coal bed methane

{dagger}These authors contributed equally to this work.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA and mcrA gene sequences of Methanolobus zinderi sp. nov. SD1T are EU711413T and EU715818T, respectively.

Graphs showing the effects of temperature, Na+, Mg2+ and pH on the growth of strain SD1T are available with the online version of this paper.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2009 by the International Union of Microbiological Societies.