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Int J Syst Evol Microbiol 59 (2009), 1064-1069; DOI  10.1099/ijs.0.003772-0
© 2009 International Union of Microbiological Societies

Methanolobus zinderi sp. nov., a methylotrophic methanogen isolated from a deep subsurface coal seam

Sebastian N. Doerfert1,{dagger}, Matthew Reichlen1,{dagger}, Prabha Iyer2, Mingyu Wang1 and James G. Ferry1

1 Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802, USA
2 J. Craig Venter Institute, Rockville, MD 20850, USA

Correspondence
James G. Ferry
jgf3{at}psu.edu

A methanogenic organism from the domain Archaea (SD1T) was isolated from saline water released from a coal seam located 926 m below the surface via a methane-producing well near Monroe, Louisiana, USA. Growth and methanogenesis were supported with methanol, monomethylamine, dimethylamine or trimethylamine, but not with dimethylsulfide, formate, acetate or H2/CO2. Cells grew in high-salt minimal medium but growth was stimulated with yeast extract or tryptone. Cells were single, non-motile, irregular coccoids 0.5–1.0 µm in diameter and the cell wall contained protein. Conditions for the maximum rate of growth were 40–50 °C, 0.2–0.6 M NaCl, 100–≥200 mM MgCl2, and pH 7.0–8.0. The G+C content of the genomic DNA was 42±1mol %. A comparison of 16S rRNA gene sequences indicated that strain SD1T was most closely related to Methanolobus oregonensis DSM 5435T with 96 % gene sequence similarity. It is proposed that strain SD1T represents a novel species, Methanolobus zinderi sp. nov. The type strain is SD1T (=ATCC BAA-1601T=DSM 21339T).

Abbreviations: CBM, coal bed methane

{dagger}These authors contributed equally to this work.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA and mcrA gene sequences of Methanolobus zinderi sp. nov. SD1T are EU711413T and EU715818T, respectively.

Graphs showing the effects of temperature, Na+, Mg2+ and pH on the growth of strain SD1T are available with the online version of this paper.






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