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Int J Syst Evol Microbiol 59 (2009), 886-892; DOI  10.1099/ijs.0.003145-0
© 2009 International Union of Microbiological Societies

Marinobacter santoriniensis sp. nov., an arsenate-respiring and arsenite-oxidizing bacterium isolated from hydrothermal sediment

Kim M. Handley{dagger}, Marina Héry and Jonathan R. Lloyd

School of Earth, Atmospheric and Environmental Sciences, University of Manchester, Manchester M13 9PL, UK

Correspondence
Kim M. Handley
kim.handley{at}berkeley.edu

A Gram-negative, arsenate-respiring and arsenite-oxidizing marine bacterium, NKSG1T, was isolated from hydrothermal sediment at Santorini, Greece. Strain NKSG1T was a facultatively anaerobic, motile, non-spore-forming, rod-shaped bacterium. Growth occurred optimally at 35–40 °C, between pH 5.5 and 9.0 and with 0.5–16 % NaCl. Energy was conserved by the aerobic oxidation of a range of complex substrates, carbohydrates and organic acids, or anaerobically by arsenate reduction, nitrate reduction coupled to the oxidation of organic carbon or lactate fermentation. Oxidation of arsenite and anaerobic nitrate-dependent oxidation of Fe(II) were facilitated by the presence of an organic carbon source. The DNA G+C content was 58.1 mol%. The major respiratory quinone was Q-9. The significant fatty acids were 16 : 1{omega}9c, summed feature 3 (iso-15 : 0 2-OH/16 : 1{omega}7c), 16 : 0 and 18 : 1{omega}9c. Analysis of 16S rRNA gene sequences showed that strain NKSG1T fits within the phylogenetic cluster of the genus Marinobacter and is most closely related to Marinobacter koreensis DD-M3T (99.3 % similarity). The degree of relatedness with M. koreensis DSM 17924T based on DNA–DNA hybridization was 56 %. The results of a polyphasic study indicated that strain NKSG1T is a representative of a novel species within the genus Marinobacter, for which the name Marinobacter santoriniensis sp. nov. is proposed. The type strain is NKSG1T (=DSM 21262T =NCIMB 14441T=ATCC BAA-1649T). The capacity for arsenic reduction or oxidation has not been demonstrated previously for this genus.

{dagger}Present address: Earth and Planetary Science, University of California, Berkeley, California 94720, USA.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain NKSG1T is EU496088.

A supplementary table showing the electron donors that facilitate nitrate reduction by strain NKSG1T and the type strains of phylogenetically related Marinobacter species is available with the online version of this paper.






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