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Int J Syst Evol Microbiol 59 (2009), 869-873; DOI  10.1099/ijs.0.005611-0
© 2009 International Union of Microbiological Societies

Iamia majanohamensis gen. nov., sp. nov., an actinobacterium isolated from sea cucumber Holothuria edulis, and proposal of Iamiaceae fam. nov.

Midori Kurahashi1, Yukiyo Fukunaga1,2, Yayoi Sakiyama2, Shigeaki Harayama2 and Akira Yokota1

1 Institute of Molecular and Cellular Biosciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-0032, Japan
2 NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE), 2-5-8 Kazusa-kamatari, Kisarazu-shi, Chiba 292-0818, Japan

Correspondence
Midori Kurahashi
kuramido{at}iam.u-tokyo.ac.jp

A novel, Gram-positive bacterial strain, F12T, was isolated from the abdominal epidermis of a sea cucumber, Holothuria edulis, collected from seawater off the coast of Japan. According to 16S rRNA gene sequence analysis, this strain represents a novel, deep-rooting lineage within the class Actinobacteria and clusters with uncultured bacteria and Acidimicrobium ferrooxidans. Compared to species with validly published names, the highest 16S rRNA gene sequence similarity (89.8 %) was to Acidimicrobium ferrooxidans DSM 10331T. Phylogenetic analyses showed that strain F12T represents a distinct phylogenetic lineage related closely to the genus Acidimicrobium. Strain F12T contained MK-9(H6) as the major menaquinone, whilst 17 : 0, 17 : 1{omega}8c, 15 : 0 and 16 : 0 were the major cellular fatty acids. The cell-wall peptidoglycan of strain F12T was composed of meso-diaminopimelic acid as the diagnostic diamino acid, alanine and glutamic acid (1 : 2 : 1). The cell-wall sugars detected were rhamnose, mannose, arabinose, galactose and xylose. The G+C content of the DNA was 74.4 mol%. From the taxonomic data obtained in this study, the name Iamia majanohamensis gen. nov., sp. nov. is proposed for the isolate, with type strain F12T (=NBRC 102561T=DSM 19957T). The name Iamiaceae fam. nov. is also proposed for the distinct phyletic line represented by the genus Iamia.

Abbreviations: DAP, diaminopimelic acid; ML, maximum likelihood; NJ, neighbour joining; TEM, transmission electron microscopy

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain F12T is AB360448.

A supplementary figure showing the general morphology of cells of strain F12T is available with the online version of this paper.






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