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Int J Syst Evol Microbiol 59 (2009), 850-855; DOI  10.1099/ijs.0.003327-0
© 2009 International Union of Microbiological Societies

Mesorhizobium metallidurans sp. nov., a metal-resistant symbiont of Anthyllis vulneraria growing on metallicolous soil in Languedoc, France

Céline Vidal1, Clémence Chantreuil2, Odile Berge3, Lucette Mauré1, José Escarré4, Gilles Béna2, Brigitte Brunel5 and Jean-Claude Cleyet-Marel1

1 INRA, USC1242, Laboratoire des Symbioses Tropicales et Méditerranéennes, Campus International de Baillarguet TA A-82/J, F-34398, Montpellier Cedex 5, France
2 IRD, UMR 113, Laboratoire des Symbioses Tropicales et Méditerranéennes, F-34398 Montpellier, France
3 CNRS, UMR 6191, Laboratoire d'Ecologie Microbienne de la Rhizosphère et des Environnements Extrêmes, F-13108 Saint-Paul lez Durance, France
4 CNRS, UMR 5175, Centre d'Ecologie Fonctionnelle et Evolutive, 1919 Route de Mende, F-34293 Montpellier, France
5 Montpellier SupAgro, UMR 113, Laboratoire des Symbioses Tropicales et Méditerranéennes, F-34398 Montpellier, France

Correspondence
Jean-Claude Cleyet-Marel
cleyet{at}supagro.inra.fr

A polyphasic taxonomic approach was used to characterize 31 rhizobial isolates obtained from Anthyllis vulneraria, a metallicolous legume species, growing close to a zinc mine in the south of France (Saint Laurent le Minier). Comparative analysis of nearly full-length 16S rRNA gene sequences showed that these Gram-negative bacteria belonged to the genus Mesorhizobium and that they were related most closely to Mesorhizobium tianshanense ORS 2640T. The phylogenetic relationships of these isolates with other Mesorhizobium species were confirmed by sequencing and analysis of the recA and atpD genes, which were used as alternative chromosomal markers. These novel mesorhizobial strains tolerated high concentrations of heavy metals: 16–32 mM Zn and 0.3–0.5 mM Cd. DNA–DNA hybridizations revealed >73 % relatedness between the strains isolated from A. vulneraria, but only 19–33 % relatedness between these and the type strains of M. tianshanense and Mesorhizobium mediterraneum. These results, together with other phenotypic characteristics, support the conclusion that these isolates represent a single, novel species of the genus Mesorhizobium, for which the name Mesorhizobium metallidurans sp. nov. is proposed. The type strain is STM 2683T (=CFBP 7147T=LMG 24485T).

The GenBank/EMBL/DDBJ accession numbers for the sequences determined in this study are AM930381–AM930393.

A table showing GenBank accession numbers for individual sequences is available with the online version of this paper.






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