IJSEM Sign up for IJSEM eTOCs
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Figures S1 and S2.
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by van Ingen, J.
Right arrow Articles by van Soolingen, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by van Ingen, J.
Right arrow Articles by van Soolingen, D.
Agricola
Right arrow Articles by van Ingen, J.
Right arrow Articles by van Soolingen, D.
Int J Syst Evol Microbiol 59 (2009), 845-849; DOI  10.1099/ijs.0.001511-0
© 2009 International Union of Microbiological Societies

Mycobacterium noviomagense sp. nov.; clinical relevance evaluated in 17 patients

Jakko van Ingen1,2, Martin J. Boeree1, Wiel C. M. de Lange1, Petra E. W. de Haas2, Adri G. M. van der Zanden3, Wouter Mijs4, Leen Rigouts5, P. N. Richard Dekhuijzen1 and Dick van Soolingen2

1 Radboud University Nijmegen Medical Center, Department of Pulmonary Diseases, Nijmegen, The Netherlands
2 National Institute for Public Health and the Environment, National Mycobacteria Reference Laboratory, Bilthoven, The Netherlands
3 Laboratory for Medical Microbiology and Public Health, Enschede, The Netherlands
4 Innogenetics N.V., Department of Diagnostics, Gent, Belgium
5 Institute of Tropical Medicine, Department of Mycobacteriology, Antwerp, Belgium

Correspondence
Jakko van Ingen
jakko.van.ingen{at}rivm.nl

Eighteen isolates of a nonchromogenic, slowly growing, non-tuberculous species of the genus Mycobacterium were cultured from respiratory specimens obtained over the last eight years from 17 patients in the Netherlands. These isolates were grouped because they revealed a unique 16S rRNA gene sequence and were related to Mycobacterium xenopi. None of the 17 patients met the American Thoracic Society diagnostic criteria for non-tuberculous mycobacterial disease, which distinguishes the novel isolates from the related species, M. xenopi. A polyphasic taxonomic approach, including identification by biochemical and phenotypical analysis, hsp65 gene sequencing and PCR restriction enzyme pattern analysis, and sequence analyses of the rpoB gene and 16S–23S internal transcribed spacer supported the separate species status of the novel isolates. The name Mycobacterium noviomagense sp. nov. is proposed for the novel strains. The type strain is NLA000500338T (=DSM 45145T=CIP 109766T). A more distinctive taxonomy of NTM is a prerequisite for the assessment of their clinical relevance.

Abbreviations: ATS, American Thoracic Society; ITS, internal transcribed spacer; NTM, non-tuberculous mycobacteria

The GenBank/EMBL/DDBJ accession numbers 16S rRNA, 16S–23S ITS 1 and 2, hsp65 and rpoB gene sequences of strain NLA000500338T are EU239955, EU439248, EU439249, EU600390 and EU810775, respectively

Phylogenetic trees based on hsp65 and rpoB gene sequences of selected mycobacterial species are available as supplementary material with the online version of this paper.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2009 by the International Union of Microbiological Societies.