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Int J Syst Evol Microbiol 59 (2009), 828-834; DOI  10.1099/ijs.0.003640-0
© 2009 International Union of Microbiological Societies

Actinomadura keratinilytica sp. nov., a keratin-degrading actinobacterium isolated from bovine manure compost

Aaron A. Puhl1, L. Brent Selinger2, Tim A. McAllister1 and G. Douglas Inglis1

1 Agriculture and Agri-Food Canada Research Centre, 5403 1st Avenue S, Lethbridge, AB T1J 4B1, Canada
2 Department of Biological Sciences, University of Lethbridge, 4401 University Drive, Lethbridge, AB T1K 3M4, Canada

Correspondence
G. Douglas Inglis
inglisd{at}agr.gc.ca

A novel keratinolytic actinobacterium, strain WCC-2265T, was isolated from bovine hoof keratin ‘baited’ into composting bovine manure from southern Alberta, Canada, and subjected to phenotypic and genotypic characterization. Strain WCC-2265T produced well-developed, non-fragmenting and extensively branched hyphae within substrates and aerial hyphae, from which spherical spores possessing spiny cell sheaths were produced in primarily flexuous or straight chains. The cell wall contained meso-diaminopimelic acid, whole-cell sugars were galactose, glucose, madurose and ribose, and the major menaquinones were MK-9(H6), MK-9(H8), MK-9(H4) and MK-9(H2). These characteristics suggested that the organism belonged to the genus Actinomadura and a comparative analysis of 16S rRNA gene sequences indicated that it formed a distinct clade within the genus. Strain WCC-2265T could be differentiated from other species of the genus Actinomadura by DNA–DNA hybridization, morphological and physiological characteristics and the predominance of iso-C16 : 0, iso-C17 : 0 and 10-methyl C17 : 0 fatty acids. The broad range of phenotypic and genetic characters supported the suggestion that this organism represents a novel species of the genus Actinomadura, for which the name Actinomadura keratinilytica sp. nov. is proposed; the type strain is strain WCC-2265T (=DSM 45195T=CCUG 56181T).

Abbreviations: SEM, scanning electron microscopy

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strain WCC-2265T and Actinomadura rubrobrunea DSM 43750T are EU637009 and EU637008, respectively.

A table showing the fatty acid profiles of strain WCC-2265T and related strains and a figure showing the growth of strain WCC-2265T on keratin agar are available with the online version of this paper.






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Copyright © 2009 by the International Union of Microbiological Societies.