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Int J Syst Evol Microbiol 59 (2009), 800-805; DOI  10.1099/ijs.0.003731-0
© 2009 International Union of Microbiological Societies

Methanofollis ethanolicus sp. nov., an ethanol-utilizing methanogen isolated from a lotus field

Hiroyuki Imachi1, Sanae Sakai1, Hiroyuki Nagai2, Takashi Yamaguchi2 and Ken Takai1

1 Subground Animalcule Retrieval (SUGAR) Program, Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Kanagawa 237-0061, Japan
2 Department of Environmental Systems Engineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan

Correspondence
Hiroyuki Imachi
imachi{at}jamstec.go.jp

A novel methane-producing archaeon, designated strain HASUT, was isolated from a lotus field. Cells were Gram-negative, non-motile, irregular cocci, 2–3 µm in diameter, and occurred singly. Growth was observed at 15–40 °C (optimum, 37 °C) and pH 6.5–7.5 (optimum, pH 7.0). The G+C content of the genomic DNA was 60.9 mol%. Strain HASUT utilized ethanol, 1-propanol, 1-butanol, hydrogen and formate for growth and methane production. It converted ethanol to methane and acetate. Based on comparative 16S rRNA gene sequence analysis, strain HASUT was shown to be affiliated with the genus Methanofollis. It was related most closely to the type strain of Methanofollis liminatans (96.1 % 16S rRNA gene sequence similarity). Based on phylogenetic analysis and phenotypic characteristics, strain HASUT is considered to represent a novel species of the genus Methanofollis, for which the name Methanofollis ethanolicus sp. nov. is proposed. The type strain is HASUT (=NBRC 104120T=JCM 15103T=DSM 21041T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain HASUT is AB371073.






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