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Lacticigenium naphtae gen. nov., sp. nov., a halotolerant and motile lactic acid bacterium isolated from crude oil

NITE Biological Resource Center, National Institute of Technology and Evaluation, 2-5-8, Kazusakamatari, Kisarazu, Chiba 292-0818, Japan

Correspondence
Takao Iino
iino-takao{at}nite.go.jp

A novel lactic acid bacterium, strain MIC1-18^T, was isolated from crude oil collected at an oil–water well in Akita, Japan. Cells of strain MIC1-18^T were found to be facultatively anaerobic, mesophilic, neutrophilic, Gram-negative, non-sporulating, motile by means of peritrichous flagella and oval rods, 1.8–2.5 µm long. Optimum growth was observed at 30 °C, pH 7.0 and 3 % (w/v) NaCl. Strain MIC1-18^T produced acid from L-arabinose, ribose, glucose, fructose, mannose, N-acetylglucosamine, amygdalin, arbutin, salicin, cellobiose, maltose, sucrose, trehalose, gentiobiose and 5-ketogluconate. L-Lactic acid was the major end product from glucose. The major cellular fatty acid was C_{16 : 1}7c. The cell-wall murein type was A4 containing Lys–Glu. The G+C content of the genomic DNA was 37.8 mol%. Phylogenetic analysis based on the 16S rRNA gene revealed that strain MIC1-18^T was accommodated as a member of the lactic acid bacteria of the low-G+C content Gram-positive bacteria; the closest neighbour of this organism was Atopococcus tabaci CCUG 48253^T, with only 90.0 % sequence similarity. On the basis of the phenotypic features and phylogenetic position, a novel genus and species, Lacticigenium naphtae gen. nov., sp. nov., are proposed for strain MIC1-18^T (=NBRC 101988^T=DSM 19658^T).

A supplementary figure showing phase-contrast and transmission electron micrographs of cells of strain MIC1-18^T is available with the online version of this paper.