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Int J Syst Evol Microbiol 59 (2009), 698-704; DOI  10.1099/ijs.0.001990-0
© 2009 International Union of Microbiological Societies

Description of Enterovibrio nigricans sp. nov., reclassification of Vibrio calviensis as Enterovibrio calviensis comb. nov. and emended description of the genus Enterovibrio Thompson et al. 2002

Javier Pascual1,2, M. Carmen Macián3, David R. Arahal2,3, Esperanza Garay2,3 and María J. Pujalte1,2

1 Instituto Cavanilles de Biodiversidad y Biología Evolutiva (ICBiBE), Universidad de Valencia, Valencia, Spain
2 Departamento de Microbiología y Ecología, Universidad de Valencia, Valencia, Spain
3 Colección Española de Cultivos Tipo (CECT), Universidad de Valencia, Valencia, Spain

Correspondence
María J. Pujalte
maria.j.pujalte{at}uv.es

Eleven strains of halophilic, facultative anaerobes isolated from healthy and diseased Dentex dentex and Sparus aurata (bony fishes) cultured in Spanish Mediterranean fisheries have been studied by a polyphasic approach that included a wide phenotypic characterization, DNA–DNA hybridization and phylogenetic analysis using 16S rRNA, recA and rpoD gene sequences. All strains were phylogenetically related to Enterovibrio species and Vibrio calviensis. On the basis of sequence analysis and DNA–DNA hybridization data, eight of the strains were identified as Enterovibrio coralii. The remaining three strains formed a tight, independent clade in all sequence analyses and showed less than 70 % DNA–DNA hybridization with strains of the closest Enterovibrio species, from which they could be differentiated by several phenotypic traits. We conclude that these three strains represent a novel species in the genus Enterovibrio and we thus propose the name Enterovibrio nigricans sp. nov., with strain DAl 1-1-5T (=CECT 7320T =CAIM 661T) as the type strain. In addition, we propose the reclassification of Vibrio calviensis Denner et al. 2002 as Enterovibrio calviensis comb. nov. (type strain RE35/F12T =CIP 107077T =DSM 14347T =CECT 7414T) and we provide an emended description of the genus Enterovibrio.

Abbreviations: ML, maximum likelihood; MP, maximum parsimony; NJ, neighbour joining

The GenBank/EMBL/DDBJ accession numbers for the rpoD, recA and 16S rRNA gene sequences determined in this study are AM942047–AM942062, AM942063–AM942078 and AM942722–AM942732, respectively, as detailed in Table 1.

Photographs of colonies of strain DAl 1-1-5T, ML and MP trees based on 16S rRNA, recA and rpoD gene sequences and a concatenated dataset, growth responses and cellular fatty acid profiles of individual strains and detailed DNA–DNA hybridization results are available as supplementary material with the online version of this paper.






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