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Int J Syst Evol Microbiol 59 (2009), 654-657; DOI  10.1099/ijs.0.003210-0
© 2009 International Union of Microbiological Societies

Cellulophaga tyrosinoxydans sp. nov., a tyrosinase-producing bacterium isolated from seawater

Hyung-Yeel Kahng1, Bok Sil Chung2,3, Dong-Heon Lee1, Jae Sung Jung4, Jin Ho Park2 and Che Ok Jeon3

1 Department of Environmental Education, Sunchon National University, Sunchon 540-742, Republic of Korea
2 Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea
3 Department of Life Science, Chung-Ang University, Seoul 156-756, Republic of Korea
4 Department of Life Science, Sunchon National University, Sunchon 540-742, Republic of Korea

Correspondence
Che Ok Jeon
cojeon{at}cau.ac.kr

An aerobic, gliding, yellow-pigmented bacterium lacking flagella and showing strong tyrosinase activity, designated strain EM41T, was isolated from seawater on the eastern coast of Jeju Island in Korea. Growth was observed at 15–35 °C (optimum, 25–30 °C) and at pH 6.5–9.0 (optimum, pH 7.0–8.5). Cells were Gram-negative, negative for flexirubin pigments and catalase- and oxidase-positive. The G+C content of the genomic DNA was 33.5 mol% and the major respiratory quinone was menaquinone-6 (MK-6). Comparative 16S rRNA gene sequence analysis showed that strain EM41T formed a distinct phyletic line within the genus Cellulophaga with a 100 % bootstrap value and was most closely related to Cellulophaga pacifica KMM 3664T (97.0 % sequence similarity). The level of DNA–DNA relatedness between strain EM41T and C. pacifica KMM 3664T was about 17.8 %. On the basis of phenotypic and genotypic data, strain EM41T is considered to represent a novel species of the genus Cellulophaga, for which the name Cellulophaga tyrosinoxydans sp. nov. is proposed. The type strain is EM41T (=KCTC 22297T=DSM 21164T).


The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain EM41T is EU443205.

A transmission electron micrograph of cells of strain EM41T is available with the online version of this paper.







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