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Int J Syst Evol Microbiol 59 (2009), 622-630; DOI  10.1099/ijs.0.004390-0
© 2009 International Union of Microbiological Societies

Cystofilobasidium lacus-mascardii sp. nov., a basidiomycetous yeast species isolated from aquatic environments of the Patagonian Andes, and Cystofilobasidium macerans sp. nov., the sexual stage of Cryptococcus macerans

Diego Libkind1, Mário Gadanho2, Maria van Broock1 and José Paulo Sampaio2

1 Laboratorio de Microbiología Aplicada y Biotecnología, Universidad Nacional del Comahue, Centro Regional Universitario Bariloche (CRUB), CONICET (Consejo Nacional de Investigaciones Científicas y Tecnológicas), Bariloche, Río Negro, Argentina
2 Centro de Recursos Microbiológicos, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal

Correspondence
José Paulo Sampaio
jss{at}fct.unl.pt

Here, we report on two novel sexual basidiomycetous red yeast species of the genus Cystofilobasidium. Cystofilobasidium lacus-mascardii sp. nov. is based on sexually compatible strains isolated from Lake Mascardi, an ultraoligotrophic lake in north-western Patagonia, Argentina. Following the discovery of the first isolate of this species, additional (sexually compatible) strains were isolated using a selective medium containing erythritol as the sole source of carbon. The second novel species corresponds to the sexual state of Cryptococcus macerans. In spite of accounts over the last 20 years of sexually compatible strains of this species, the complete life has never been observed. We provide evidence of a Cystofilobasidium-like basidial stage with teliospores and slender holobasidia, based on the study of self-fertile (homothallic) and self-sterile (heterothallic) isolates of Cryptococcus macerans. A revised molecular phylogeny of the genus Cystofilobasidium is presented and the most salient features of Cystofilobasidium lacus-mascardii sp. nov. (type strain CBS 10642T =PYCC 5819T =CRUB 1046T) and Cystofilobasidium macerans sp. nov. (type strain CBS 10757T) are discussed and compared with those of the remaining species in the genus. Information on additional Patagonian isolates belonging to the Cystofilobasidiales is also included in this report.

Abbreviations: ITS, internal transcribed spacer; LSU, large subunit; MP, maximum parsimony

The GenBank/EMBL/DDBJ accession number numbers for the sequences determined in this study are EF613496–EF613498 and EU082223–EU082228 (LSU rDNA D1D2 domain) and EF613495 and EU082229–EU082233 (compete ITS region and 5.8S rDNA).






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