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Int J Syst Evol Microbiol 59 (2009), 583-588; DOI  10.1099/ijs.0.002808-0
© 2009 International Union of Microbiological Societies

Thiobacillus thiophilus sp. nov., a chemolithoautotrophic, thiosulfate-oxidizing bacterium isolated from contaminated aquifer sediments

Claudia Kellermann and Christian Griebler

Helmholtz Center Munich – German Research Center for Environmental Health, Institute of Groundwater Ecology, Ingolstaedter Landstrasse 1, D-85764 Neuherberg, Germany

Correspondence
Christian Griebler
christian.griebler{at}helmholtz-muenchen.de

Strain D24TNT was enriched and isolated from sediment collected from a tar oil-contaminated aquifer at a former gasworks site located in Duesseldorf-Flingern, Germany. Cells of strain D24TNT were rod-shaped, non-spore-forming and stained Gram-negative. Thiosulfate was used as an electron donor. The organism was obligately chemolithoautotrophic and facultatively anaerobic, and grew with either oxygen or nitrate as electron acceptor. Growth was observed at pH values between 6.3 and 8.7 and at temperatures of –2 to 30 °C; optimum growth occurred at pH 7.5–8.3 and 25–30 °C. The DNA G+C content was 61.5 mol%. On the basis of the 16S rRNA gene sequence analysis, strain D24TNT clustered in the Betaproteobacteria and was most closely related to Thiobacillus denitrificans (97.6 %) and Thiobacillus thioparus (97.5 %). Based on the phenotypic, chemotaxonomic and phylogenetic data, strain D24TNT represents a novel species of the genus Thiobacillus, for which the name Thiobacillus thiophilus sp. nov. is proposed. The type strain is D24TNT (=DSM 19892T=JCM 15047T).

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, cbbL and cbbM gene sequences of strain D24TNT are EU685841, EU746410 and EU746411, respectively.

Figures showing growth curves of cells of strain D24TNT under aerobic and anaerobic conditions and consensus phylogenetic trees based on cbbL and cbbM genes are available as supplementary material with the online version of this paper.






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Copyright © 2009 by the International Union of Microbiological Societies.