HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Zhao, J.-S. Articles by Hawari, J. Search for Related Content PubMed PubMed Citation Articles by Zhao, J.-S. Articles by Hawari, J. Agricola Articles by Zhao, J.-S. Articles by Hawari, J.

Psychrilyobacter atlanticus gen. nov., sp. nov., a marine member of the phylum Fusobacteria that produces H₂ and degrades nitramine explosives under low temperature conditions

Biotechnology Research Institute, National Research Council of Canada, 6100 Royalmount Ave, Montreal, Quebec H4P 2R2, Canada

Correspondence
Jian-Shen Zhao
jian-shen.zhao{at}cnrc-nrc.gc.ca
Jalal Hawari
jalal.hawari{at}nrc.ca

A Gram-negative and obligately anaerobic marine bacterium, strain HAW-EB21^T, was isolated in a previous study from marine sediment from the Atlantic Ocean, near Halifax Harbor, Canada, and found to have the potential to degrade both hexahydro-1,3,5-trinitro-1,3,5-triazine and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine. In the present study, phylogenetic analyses showed that strain HAW-EB21^T was only distantly related to the genera Propionigenium and Ilyobacter with 6.6–7.5 % and 8.2–10.5 % dissimilarity as measured by 16S rRNA and 23S rRNA gene sequence analyses, respectively. Strain HAW-EB21^T displayed unique properties in being psychrotrophic (18.5 °C optimum) and unable to utilize any of the carbon substrates (succinate, L-tartrate, 3-hydroxybutyrate, quinate or shikimate) used for isolating members of the genera Propionigenium and Ilyobacter. Strain HAW-EB21^T utilized glucose, fructose, maltose, N-acetyl-D-glucosamine, citrate, pyruvate, fumarate and Casitone as carbon sources and produced H₂ and acetate as the major fermentation products. Cells grown at 10 °C produced C_{15 : 1} (30 %), C_{16 : 1}7 (15 %) and C_{16 : 0} (16 %) as major membrane fatty acids. The novel strain had a genomic DNA G+C content of 28.1 mol%, lower than the values of the genera Ilyobacter and Propionigenium. Based on the present results, the novel isolate is suggested to be a member of a new genus for which the name Psychrilyobacter atlanticus gen. nov., sp. nov. is proposed. The type strain of the type species is HAW-EB21^T (=DSM 19335^T=JCM 14977^T).

The GenBank/EMBL/DDBJ accession numbers for the 16S and 23S rRNA gene sequences of strain HAW-EB21^T are AY579753 and EF426680, respectively.