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Int J Syst Evol Microbiol 59 (2009), 336-340; DOI  10.1099/ijs.0.000737-0
© 2009 International Union of Microbiological Societies

Shewanella vesiculosa sp. nov., a psychrotolerant bacterium isolated from an Antarctic coastal area

Núria Bozal, Ma Jesús Montes, David Miñana-Galbis, Angeles Manresa and Elena Mercadé

Laboratori de Microbiologia, Facultat de Farmacia, Universitat de Barcelona, Av. Joan XXIII s/n, 08028 Barcelona, Spain

Correspondence
Elena Mercadé
mmercade{at}ub.edu

Two strains of psychrotolerant bacteria, designated M7T and M5, isolated from Antarctic coastal marine environments were studied to determine their taxonomic position. The organisms comprised Gram-negative, rod-shaped, facultatively anaerobic cells that were motile by means of single polar flagella. Neither of the bacterial isolates had a requirement for Na+. These two psychrotolerant strains grew at temperatures ranging from –4 to 30 °C. Both strains were capable of producing H2S from thiosulfate and were able to use sodium nitrate and trimethylamine N-oxide as terminal electron acceptors during anaerobic growth. 16S rRNA gene sequence analysis placed M7T and M5 within the genus Shewanella; the strains showed the highest similarity (99.9 and 99.2 % respectively) with respect to the type strains of Shewanella livingstonensis and Shewanella frigidimarina. However the levels of gyrB sequence similarity between strain M7T and the type strains of S. livingstonensis and S. frigidimarina were 87.6 and 87.4 %, respectively. DNA–DNA hybridization experiments performed between the Antarctic isolate M7T and S. livingstonensis LMG 19866T and S. frigidimarina LMG 19475T revealed levels of relatedness of 32 and 35 %, respectively. Strain M5 showed 100 % DNA relatedness with respect to strain M7T. The DNA G+C content of these bacteria was 42 mol%. Several phenotypic characteristics, the cellular fatty acid compositions and the quinone content of strains M7T and M5 served to differentiate them from related shewanellae. On the basis of the data from this polyphasic taxonomic study, M7T and M5 constitute a single genospecies. They represent a novel species of the genus Shewanella, for which the name Shewanella vesiculosa sp. nov. is proposed. The type strain is M7T (=LMG 24424T =CECT 7339T).

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains M7T and M5 are AM980877 and AM980878, respectively, and those for the gyrB gene sequences of strains M7T and S. livingstonensis NF22T are EU702750 and EU702751, respectively.

Transmission electron micrographs of ultrathin sections of cells of strain M7T and details of the cellular fatty acid compositions for strains M7T and M5 are presented as supplementary material available with the online version of this paper.






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Copyright © 2009 by the International Union of Microbiological Societies.