HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Table and Figures Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Glaeser, S. P. Articles by Glaeser, J. Search for Related Content PubMed PubMed Citation Articles by Glaeser, S. P. Articles by Glaeser, J. Agricola Articles by Glaeser, S. P. Articles by Glaeser, J.

Novosphingobium acidiphilum sp. nov., an acidophilic salt-sensitive bacterium isolated from the humic acid-rich Lake Grosse Fuchskuhle

¹ Institut für Mikrobiologie und Molekularbiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany
² Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany
³ Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität Wien, A-1210 Wien, Austria

Correspondence
Jens Glaeser
Jens.Glaeser{at}mikro.bio.uni-giessen.de

A yellow- to orange-pigmented, Gram-negative, rod-shaped, motile and non-spore-forming bacterium, strain FSW06-204d^T, was isolated from subsurface water of the acidic bog lake, Lake Grosse Fuchskuhle (Brandenburg, Germany). Optimum growth of this strain occurred over a pH range from 5.5 to 6.0 and the growth rate strongly decreased at pH values above 6.5. In addition, the strain exhibited a low tolerance towards NaCl and grew only at a NaCl concentration of up to 0.5 %. 16S rRNA gene sequence analysis of strain FSW06-204d^T showed the highest sequence similarity to Novosphingobium hassiacum W-51^T (96.7 %) and formed a distinct cluster with Novosphingobium nitrogenifigens DSM 19370^T (96.4 %) within the genus Novosphingobium. Strain FSW06-204d^T shared a 21 bp signature gap with the latter species, a feature that is absent in all other members of the family Sphingomonadaceae. DNA–DNA hybridization of strain FSW06-204d^T and N. nitrogenifigens DSM 19370^T showed a low relatedness value of 24 % (reciprocal: 39 %). The major respiratory quinone was ubiquinone Q-10 (91 %) and the predominant fatty acid was C_{18 : 1}7c (43.3 %). Two characteristic 2-hydroxy fatty acids, C_{14 : 0} 2-OH (8.1 %) and C_{15 : 0} 2-OH (6.5 %), were abundant. Polar lipids consisted mainly of phosphatidyldimethylethanolamine and phosphatidylethanolamine; however, only moderate amounts of sphingoglycolipids were present and phosphatidylcholine was lacking. Characterization by 16S rRNA gene sequence, physiological features, pigment analysis and polyamine, ubiquinone, polar lipid and fatty acid contents revealed that strain FSW06-204d^T represents a novel species of the genus Novosphingobium within the class Alphaproteobacteria. The name Novosphingobium acidiphilum sp. nov. is proposed for this acidophilic and salt-sensitive species with the type strain FSW06-204d^T (=DSM 19966^T=CCM 7496^T=CCUG 55538^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain FSW06-204d^T is EU336977.

The results of a comparative pigment analysis for strain FSW06-204d^T and related strains are presented in a supplementary table available with the online version of this paper. Supplementary figures showing a maximum-parsimony phylogenetic tree based on 16S rRNA gene sequences, the polar lipid profile of strain FSW06-204d^T and the results of a pigment analysis for the novel strain are also available.