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Int J Syst Evol Microbiol 59 (2009), 319-322; DOI  10.1099/ijs.0.002626-0
© 2009 International Union of Microbiological Societies

Prevotella falsenii sp. nov., a Prevotella intermedia-like organism isolated from monkey dental plaque

Mitsuo Sakamoto1,{dagger}, Hidefumi Kumada2,{dagger}, Nobushiro Hamada2, Yusuke Takahashi2, Masaaki Okamoto3, Mohammad Abdul Bakir1 and Yoshimi Benno1

1 Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama 351-0198, Japan
2 Department of Oral Microbiology, Kanagawa Dental College, 82 Inaoka-cho, Yokosuka, Kanagawa 238-8580, Japan
3 Tsurumi University, School of Dental Medicine, Tsurumi, Yokohama, Kanagawa 230-8501, Japan

Correspondence
Mitsuo Sakamoto
sakamoto{at}jcm.riken.jp

Eight anaerobic, pigmented, non-spore-forming, Gram-negative, rod-shaped strains isolated from monkey oral cavities were characterized phenotypically and chemotaxonomically and their phylogenetic positions were determined using 16S rRNA gene sequence analysis. The 16S rRNA gene sequence analysis showed that these isolates represent a single species of the genus Prevotella. These strains were most closely related to Prevotella intermedia ATCC 25611T, with 95.0 % 16S rRNA gene sequence similarity. The next most closely related species were Prevotella pallens and Prevotella nigrescens (92.7 and 92.1 % similarity to the respective type strains). The phenotypic and biochemical characteristics of the isolates were the same as those of P. intermedia JCM 12248T and P. nigrescens JCM 12250T. The isolates could be differentiated from P. pallens JCM 11140T on the basis of mannose fermentation and {alpha}-fucosidase activity. The isolates could not be distinguished from P. intermedia or P. nigrescens using conventional biochemical tests. DNA–DNA hybridization experiments revealed the genomic distinctiveness of these eight strains with respect to P. pallens JCM 11140T, P. intermedia JCM 12248T and P. nigrescens JCM 12250T. On the basis of these data, strains 04013, 04021, 04043, 04052T, 0406, 04113, 04111 and 04161 represent a novel Prevotella species, for which the name Prevotella falsenii sp. nov. is proposed. The type strain is 04052T (=JCM 15124T =CCUG 56137T).

{dagger}These authors contributed equally to this work.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains 04013, 04021, 04043, 04052T, 0406, 04113, 04111 and 04161 are AB429501–AB429508, respectively.

The sequence of the primer pair for P. intermedia and alignment of the 16S rRNA gene sequences of related species are presented in a supplementary figure available with the online version of this paper.






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