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Hydrogenoanaerobacterium saccharovorans gen. nov., sp. nov., isolated from H₂-producing UASB granules

¹ State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China
² Graduate School of Chinese Academy of Sciences, Beijing, PR China

Correspondence
Xiuzhu Dong
dongxz{at}sun.im.ac.cn

Two strictly anaerobic bacterial strains, designated SW512^T and W72, were isolated from a laboratory-scale H₂-producing up-flow anaerobic sludge blanket (UASB) reactor. Cells were Gram-stain-negative, non-motile and 0.3–0.4x2.0–14.5 µm; they did not form spores. Both strains grew at 24–45 °C (no growth at 22 °C or 46 °C), with optimum growth at 37 °C. The pH range for growth was 4.5–9.0 (no growth at pH4.2 or pH9.3), with optimum growth at pH 6.5–7.0. Several kinds of mono-, di- and oligosaccharides supported growth. The main end products of glucose fermentation were ethanol, acetate, hydrogen and carbon dioxide (according to the equation 1 mol glucose1.1 mol acetate+0.6 mol ethanol+2.6 mol H₂+1.6 mol CO₂). The DNA G+C contents of strains SW512^T and W72 were 41.9±0.5 and 42.8±0.4 mol% (T_m method), respectively. Phylogenetic analysis based on 16S rRNA gene sequence similarities indicated that the two strains represent a new phyletic sublineage within the family ‘Ruminococcaceae’, with <91.3 % 16S rRNA gene sequence similarity to recognized species. On the basis of the polyphasic evidence in this study, it is proposed that the two strains represent a novel species in a new genus, for which the name Hydrogenoanaerobacterium saccharovorans gen. nov., sp. nov. is proposed; the type strain of the type species is SW512^T (=AS 1.5070^T=JCM 14861^T).

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of Hydrogenoanaerobacterium saccharovorans sp. nov. SW512^T and W72 are EU158190 and EU170433, respectively.

A dendrogram constructed using the UPGMA method based on 16S rRNA gene sequence similarities between strain SW512^T and related strains is available with the online version of this paper.