IJSEM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kallimanis, A.
Right arrow Articles by Koukkou, A. I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kallimanis, A.
Right arrow Articles by Koukkou, A. I.
Agricola
Right arrow Articles by Kallimanis, A.
Right arrow Articles by Koukkou, A. I.
Int J Syst Evol Microbiol 59 (2009), 275-279; DOI  10.1099/ijs.0.000984-0
© 2009 International Union of Microbiological Societies

Arthrobacter phenanthrenivorans sp. nov., to accommodate the phenanthrene-degrading bacterium Arthrobacter sp. strain Sphe3

A. Kallimanis1, K. Kavakiotis1, A. Perisynakis1, C. Spröer2, R. Pukall2, C. Drainas1 and A. I. Koukkou1

1 Sector of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece
2 DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstr. 7b, D-38124 Braunschweig, Germany

Correspondence
A. I. Koukkou
akukku{at}cc.uoi.gr

A novel phenanthrene-degrading bacterium, designated strain Sphe3T, was isolated from a creosote-contaminated soil in Greece. Cells were non-motile, Gram-positive, aerobic, and rod- to coccus-shaped. The strain was isolated on the basis of formation of a clear zone on agar plates sprayed with phenanthrene. Optimal growth occurred at 30 °C. The G+C content of the DNA was 65.7 mol%. The polar lipid pattern of strain Sphe3T consisted of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The dominant fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-C16 : 0, C16 : 0 and anteiso-C17 : 0, representing >86 % of the total fatty acids. The predominant isoprenoid quinone of strain Sphe3T was menaquinone-8 (MK-8). Based on 16S rRNA gene sequence analysis, strain Sphe3T showed 99 and 98.9 % similarity to the type strains of Arthrobacter oxydans and Arthrobacter polychromogenes, respectively. Strain Sphe3T showed 91 % similarity to homologues of A. oxydans and A. polychromogenes based on recA gene sequence analysis. Based on 16S rRNA and recA gene sequence analysis and DNA–DNA hybridization analysis, as well as physiological and chemotaxonomic characteristics, it is concluded that strain Sphe3T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter phenanthrenivorans sp. nov. is proposed. The type strain is Sphe3T (=DSM 18606T =LMG 23796T).

Abbreviations: PAHs, polycyclic aromatic hydrocarbons

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA and recA gene sequences of strain Sphe3T are AM176541 and AM931439, respectively.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2009 by the International Union of Microbiological Societies.