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Proposal of Sinomonas flava gen. nov., sp. nov., and description of Sinomonas atrocyanea comb. nov. to accommodate Arthrobacter atrocyaneus

¹ Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China
² Quality and Standards Institute of Agricultural Products, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, PR China
³ Biotoxin Department of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, PR China

Correspondence
Ren Lai
rlai72{at}njau.edu.cn
or
zhouy81{at}yahoo.com.cn

A novel actinomycete strain, designated CW 108^T, was isolated from a forest soil in Anhui Province, China. The cells were strictly aerobic, non-motile, bent rods. The strain grew optimally at 30–37 °C and pH 6.0–8.0. Chemotaxonomically, the peptidoglycan type was A3, the cell-wall sugars contained galactose, mannose and ribose, the polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylmonomethylethanolamine, the major fatty acids were ai-C_{15 : 0}, i-C_{15 : 0} and ai-C_{17 : 0} and the predominant respiratory quinone was MK-9(H₂), with a moderate amount of MK-8(H₂) and a minor amount of MK-6(H₂). The G+C content of the genomic DNA was 71.1 mol%. Phylogenetic analysis showed that strain CW 108^T formed a deeply separated lineage in the family Micrococcaceae with Arthrobacter atrocyaneus DSM 20127^T. The 16S rRNA gene sequence similarity between strain CW 108^T and Arthrobacter atrocyaneus DSM 20127^T was 99.4 %; sequence similarities between these strains and representatives of other species in the family Micrococcaceae with validly published names were lower than 95.2 %. DNA–DNA hybridization showed a level of relatedness of 52.2 % between the two neighbours. Based on the results of our polyphasic taxonomic study, strain CW 108^T and Arthrobacter atrocyaneus DSM 20127^T should be assigned to two different species of a novel genus within the family Micrococcaceae, for which the names Sinomonas flava gen. nov., sp. nov. and Sinomonas atrocyanea comb. nov. are proposed. The type strain of Sinomonas flava is CW 108^T (=LMG 24447^T =KCTC 19388^T =CCTCC AB 207194^T) and the type strain of Sinomonas atrocyanea is DSM 20127^T =JCM 1329^T =CGMCC 1.1891^T =ATCC 13752^T.

Electron micrographs of cells of strain CW 108^T, photographs of colonies of strains CW 108^T and DSM 20127^T, a 16S rRNA gene sequence-based maximum-parsimony tree and a 2D TLC plate of polar lipids of strain CW 108^T are available as supplementary material with the online version of this paper.