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Int J Syst Evol Microbiol 59 (2009), 229-233; DOI  10.1099/ijs.0.65616-0
© 2009 International Union of Microbiological Societies

Desulfovibrio paquesii sp. nov., a hydrogenotrophic sulfate-reducing bacterium isolated from a synthesis-gas-fed bioreactor treating zinc- and sulfate-rich wastewater

Bernd H. G. W. van Houten1,{dagger}, Roel J. W. Meulepas1, Wim van Doesburg1, Hauke Smidt1, Gerard Muyzer2 and Alfons J. M. Stams1

1 Laboratory of Microbiology, Wageningen University, Dreijenplein 10, Gebouw 316, 6703 HB Wageningen, The Netherlands
2 Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands

Correspondence
Alfons J. M. Stams
fons.stams{at}wur.nl

A hydrogenotrophic, sulfate-reducing bacterium, designated strain SB1T, was isolated from sulfidogenic sludge of a full-scale synthesis-gas-fed bioreactor used to remediate wastewater from a zinc smelter. Strain SB1T was found to be an abundant micro-organism in the sludge at the time of isolation. Hydrogen, formate, pyruvate, lactate, malate, fumarate, succinate, ethanol and glycerol served as electron donors for sulfate reduction. Organic substrates were incompletely oxidized to acetate. 16S rRNA gene sequence analysis showed that the closest recognized relative to strain SB1T was Desulfovibrio gigas DSM 1382T (97.5 % similarity). The G+C content of the genomic DNA of strain SB1T was 62.2 mol%, comparable with that of Desulfovibrio gigas DSM 1382T (60.2 mol%). However, the level of DNA–DNA relatedness between strain SB1T and Desulfovibrio gigas DSM 1382T was only 56.0 %, indicating that the two strains are not related at the species level. Strain SB1T could also be differentiated from Desulfovibrio gigas based on phenotypic characteristics, such as major cellular fatty acid composition (anteiso-C15 : 0, iso-C14 : 0 and C18 : 1 cis 9) and substrate utilization. Strain SB1T is therefore considered to represent a novel species of the genus Desulfovibrio, for which the name Desulfovibrio paquesii sp. nov. is proposed. The type strain is SB1T (=DSM 16681T=JCM 14635T).

Abbreviations: DGGE, denaturing gradient gel electrophoresis; rep-PCR, repetitive enterobacterial palindromic PCR

{dagger}Present address: Institute of Rural Sciences, Aberystwyth University, Llanbadarn Fawr, Aberystwyth SY23 3AL, UK.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strain SB1T and Desulfovibrio gigas DSM 1382T are AY726757 and DQ447183, respectively.

A rep-PCR pattern comparison of strain SB1T and Desulfovibrio strains L3 and L7 is available as supplementary material with the online version of this paper.






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